2025 U.S. Open Cup
The 2025 Lamar Hunt U.S. Open Cup is the 110th edition of the U.S. Open Cup, the knockout domestic cup competition of American soccer. It is organized by the United States Soccer Federation and will comprise 96 teams, 64 teams from all three professional tiers of the United States soccer league system and 32 amateur teams in the "Open Division". The competition is scheduled to begin on March 18 and culminate in the final on October 1. Los Angeles FC is the current champion, having won the 2024 edition but will not be defending the trophy.
The full format was announced on January 28, 2025. Major League Soccer (MLS) will send 16 teams who enter in the Round of 32; teams that are playing in the 2025 CONCACAF Champions Cup are excluded from the competition. Instead, eight affiliated MLS Next Pro reserve teams and two independent teams will participate beginning in the first round. MLS previously sent eight teams in the 2024 edition of the U.S. Open Cup and ten MLS Next Pro teams after it had attempted to withdraw all of its senior teams from the competition and was rebuked by U.S. Soccer. The USL Championship will send all 24 of its teams, with the highest sixteen rated teams entering the Third Round and the eight lower-seeded teams entering in the first round alongside the 14 teams from USL League One. The 32 amateur teams will enter in the first round.
Of the 27 American clubs in MLS, 24 will participate in the U.S. Open Cup directly or be represented by their MLS Next Pro squad. The 16 direct participants were determined by their non-qualification for other competitions or their placement in the 2024 Supporters' Shield standings. Atlanta United FC, Colorado Rapids, and expansion San Diego FC will not have any representation from their club in the 2025 U.S. Open Cup. Defending champions Los Angeles FC will be among the teams represented by their MLS Next Pro affiliate instead of the first team. Half of the MLS entrants in the Fourth Round will host their matches, while the rest will play away. The MLS Next Pro teams will be able to field players from their senior MLS team's supplemental roster via loans. U.S. Soccer also adopted new rules for the competition that restricts the use of alternative venues and has random draws for hosting beginning in the Round of 16.
Schedule
Teams
Table
- Bold denotes team is still active in the tournament.
- $: Winner of $50,000 bonus for advancing the furthest in the competition from their respective divisions.
- $$: Winner of $250,000 for being the runner-up in the competition.
- $$$: Winner of $600,000 for winning the competition.
Map
New York Shockers
Harbor City FC
Soda City FC
Boston
Chattanooga
Charlotte
Dallas
Denver
Los Angeles
Louisville
Miami
Naples
NYC
Philadelphia
Phoenix
San Francisco Bay Area
Seattle
Tulsa
Washington D.C.
Locations of teams in the tournament proper.
MLS
USLC
Division 3 (MLSNP/USL1)- Open Division (USL2/NPSL)
- Open Division (USASA/USSSA)
- Multiple Teams
Metro areas with multiple teams:
- Boston: New England Revolution (MLS), CD Faialense (BSSL)
- Chattanooga: Chattanooga FC (MLSNP), Chattanooga Red Wolves SC (USL1)
- Charlotte: Charlotte FC (MLS), Charlotte Independence (USL1)
- Dallas: FC Dallas (MLS), Foro SC (UPSL)
- Denver: Flatirons FC (USL2), Harpos FC (CPL)
- Los Angeles: Orange County SC (USLC), Los Angeles FC 2 (MLSNP), Ventura County FC (MLSNP), Laguna United FC (UPSL), Ventura County Fusion (USL2)
- Louisville: Louisville City FC (USLC), Southern Indiana FC (UPSL)
- Miami: Inter Miami CF II (MLSNP), Miami United FC (UPSL), Miami FC (USLC)
- Naples: FC Naples (USL1), Naples United FC (NPSL)
- New York City: New York City FC (MLS), New York Red Bulls (MLS), Westchester SC (USL1), FC Motown (NPSL), New York Pancyprian-Freedoms (EPSL), NY Renegades FC (UPSL)
- Philadelphia: Philadelphia Union (MLS), West Chester United SC (USLPA)
- Phoenix: Phoenix Rising FC (USLC), FC Arizona (NPSL)
- San Francisco Bay Area: San Jose Earthquakes (MLS), Oakland Roots SC (USLC), El Farolito SC (NPSL), International San Francisco (SFSFL)
- Seattle: Ballard FC (USL2), Washington Athletic Club (SRATS)
- Tulsa: FC Tulsa (USLC), Tulsa Athletic (UPSL)
- Washington D.C.: D.C. United (MLS), Loudoun United FC (USLC), Virginia Dream FC (VSSL)
Number of teams by state
A total of 36 states and D.C. were represented by clubs in the U.S. Open Cup this year.
Open Cup debuts
26 teams made their Open Cup debuts in the 2025 tournament.
- MLSNP: Columbus Crew 2, FC Cincinnati 2, Los Angeles FC 2, Inter Miami CF II, Real Monarchs, Sporting Kansas City II
- USL1: AV Alta FC, FC Naples, Portland Hearts of Pine, Texoma FC, Westchester SC
- NPSL: Naples United FC, New York Shockers
- USL2: Corpus Christi FC, Flatirons FC, Little Rock Rangers, Sarasota Paradise
- Local: CD Faialense, Harbour City FC, Laguna United FC, New Jersey Alliance FC, NY Renegades FC, Soda City FC, Southern Indiana FC, Virginia Dream FC, Washington Athletic Club
Early rounds
Bracket
The first three rounds of the tournament bracket are shown below, with bold denoting the winners of each match.
Host team listed first
Bold = winner
First round
The complete draw for the first round, including match dates and times, was announced on February 6, 2025.
| March 18 | Hartford Athletic (USLC)  | 3โ0 |  New York Shockers (NPSL) | Hartford, Connecticut |
| 6:30 p.m. EDT | Report | Stadium: Trinity Health Stadium Attendance: 2,287 Referee: Johnathan Luk |
| March 18 | Sarasota Paradise (USL2)  | 1โ2 | FC Naples (USL1) | Lakewood Ranch, Florida |
| 7:00 p.m. EDT |
| Report |
| Stadium: Premier Sports Campus Attendance: 2,248 Referee: Mazdid Coric |
| March 18 | Miami FC (USLC) | 4โ1 | Naples United FC (NPSL) | Miami, Florida |
| 7:00 p.m. EDT |
| Report |
| Stadium: FIU Soccer Stadium Referee: Edson Carvajal |
| March 18 | New Jersey Alliance FC (UPSL)  | 2โ2 (a.e.t.) (2โ4 p) |  Chattanooga Red Wolves SC (USL1) | Newark, New Jersey |
| 7:00 p.m. EDT |
| Report | Stadium: Lubetkin Field Attendance: 200 Referee: Danielle Cornetta | |
| Penalties | ||||
| ||||
| March 18 | FC Motown (NPSL) | 0โ1 | Westchester SC (USL1) | Montclair, New Jersey |
| 7:30 p.m. EDT | Report | Stadium: MSU Soccer Park at Pittser Field Attendance: 534 Referee: Caleb Firl |
| March 18 | Asheville City SC (USL2)  | 0โ0 (a.e.t.) (3โ4 p) |  Greenville Triumph SC (USL1) | Asheville, North Carolina |
| 7:30 p.m. EDT | Report | Stadium: Greenwood Field Attendance: 1,000 Referee: John Rush | ||
| Penalties | ||||
| ||||
| March 18 | West Chester United SC (USLPA)  | 2โ3 |  Loudoun United FC (USLC) | Wayne, Pennsylvania |
| 7:30 p.m. EDT |
| Report | Stadium: YSC Sports Attendance: 120 Referee: Joshua Encarnaciรณn |
| March 18 | Texoma FC (USL1)  | 1โ2 (a.e.t.) | Foro SC (UPSL) | Sherman, Texas |
| 7:00 p.m. CDT |
| Report |
| Stadium: Historic Bearcat Stadium Attendance: 350 Referee: Reyes Vargas |
| March 18 | Tulsa Athletic (TLFC)  | 0โ1 | FC Tulsa (USLC) | Tulsa, Oklahoma |
| 7:30 p.m. CDT | Report |
| Stadium: Athletic Community Field at Hicks Park Referee: Rodrigo Albuquerque |
| March 18 | AV Alta FC (USL1)  | 3โ1 | Ventura County Fusion (USL2) | Lancaster, California |
| 7:30 p.m. PDT | Report |
| Stadium: Lancaster Municipal Stadium Attendance: 2,453 Referee: Felix Granados |
| March 18 | Ventura County FC (MLSNP) | 7โ0 | Laguna United FC (UPSL) | Carson, California |
| 7:30 p.m. PDT | Report | Stadium: Dignity Health Sports Park Attendance: 200 Referee: Rafael Bonilla |
| March 19 | Columbus Crew 2 (MLSNP)  | 4โ1 | NY Renegades (UPSL) | Columbus, Ohio |
| 6:00 p.m. EDT |
| Report |
| Stadium: Lower.com Field Attendance: 620 Referee: Atahan Yaya |
| March 19 | Chattanooga FC (MLSNP) | 1โ0 (a.e.t.) | Corpus Christi FC (USL2) | Chattanooga, Tennessee |
| 6:30 p.m. EDT |
| Report | Stadium: Finley Stadium Attendance: 2,075 Referee: Wes Caouette |
| March 19 | Appalachian FC (NPSL) | 0โ3 | One Knoxville SC (USL1) | Boone, North Carolina |
| 7:00 p.m. EDT | Report |
| Stadium: ASU Soccer Stadium Attendance: 2,452 Referee: Josiah Parke |
| March 19 | Richmond Kickers (USL1) | 1โ3 | Virginia Dream FC (VSSL) | Richmond, Virginia |
| 7:00 p.m. EDT |
| Report |
| Stadium: City Stadium Attendance: 1,765 Referee: John Griggs |
| March 19 | Inter Miami CF II (MLSNP) | 4โ2 | Miami United FC (UPSL) | Fort Lauderdale, Florida |
| 7:00 p.m. EDT |
| Report |
| Stadium: Chase Stadium Attendance: 1,500 Referee: Alejo Calume |
| March 19 | Sporting Kansas City II (MLSNP)  | 1โ2 |  Des Moines Menace (USL2) | Kansas City, Missouri |
| 6:30 p.m. CDT |
| Report | Stadium: Swope Soccer Village Referee: Velemir Stefanovic |
| March 19 | Lexington SC (USLC)  | 5โ0 |  Southern Indiana FC (UPSL) | Lexington, Kentucky |
| 7:30 p.m. EDT | Report | Stadium: Lexington SC Stadium Attendance: 1,835 Referee: Rogelio Alvarez |
| March 19 | Little Rock Rangers (USL2)  | 1โ0 |  Birmingham Legion (USLC) | Little Rock, Arkansas |
| 7:00 p.m. CDT |
| Report | Stadium: Episcopal Collegiate School Attendance: 1,328 Referee: Deshun Beard |
| March 19 | El Paso Locomotive FC (USLC) | 5โ1 |  Harpos FC (CPL) | El Paso, Texas |
| 7:00 p.m. MDT | Report |
| Stadium: Southwest University Park Attendance: 1,565 Referee: Melvin Rivas |
| March 19 | Real Monarchs (MLSNP)  | 1โ3 (a.e.t.) | El Farolito (NPSL) | Herriman, Utah |
| 7:30 p.m. MDT |
| Report |
| Stadium: Zions Bank Stadium Attendance: 496 Referee: Nathan Boone |
| March 19 | Monterey Bay FC (USLC) | 4โ0 | International San Francisco (SFSFL) | Seaside, California |
| 7:00 p.m. PDT | Report | Stadium: Cardinale Stadium Attendance: 968 Referee: Robert Vincze |
| March 19 | Ballard FC (USL2)  | 0โ1 (a.e.t.) | Spokane Velocity FC (USL1) | Seattle, Washington |
| 7:30 p.m. PDT | Report |
| Stadium: Interbay Stadium Attendance: 1,508 Referee: Kelsey Harms |
| March 19 | Los Angeles FC 2 (MLSNP) | 7โ1 |  FC Arizona (NPSL) | Fullerton, California |
| 7:30 p.m. PDT |
| Report |
| Stadium: Titan Stadium Referee: Steven Cardozo |
| March 20 | Portland Hearts of Pine (USL1)  | 4โ0 |  CD Faialense (BSSL) | Lewiston, Maine |
| 6:30 p.m. EDT | Report | Stadium: Franklin Athletic Complex Attendance: 3,500 Referee: Andrew Porada |
| March 20 | Long Island Rough Riders (USL2) | 2โ3 (a.e.t.) | Charlotte Independence (USL1) | Uniondale, New York |
| 7:00 p.m. EDT |
| Report | Stadium: Mitchel Athletic Complex Attendance: 719 Referee: Ignacio Guzman |
| March 20 | Forward Madison FC (USL1)  | 5โ1 |  Duluth FC (NPSL) | Madison, Wisconsin |
| 6:00 p.m. CDT | Report |
| Stadium: Breese Stevens Field Referee: Peter Bernardy |
| March 20 | South Georgia Tormenta FC (USL1)  | 3โ0 | Harbor City FC (UPSL) | Statesboro, Georgia |
| 7:00 p.m. EDT |
| Report | Stadium: Tormenta Stadium Attendance: 667 Referee: Amin Hadzic |
| March 20 | Carolina Core FC (MLSNP) | 4โ2 | Soda City FC (UPSL) | High Point, North Carolina |
| 7:30 p.m. EDT |
| Report |
| Stadium: Truist Point Attendance: 2,511 Referee: Joe Surgan |
| March 20 | FC Cincinnati 2 (MLSNP) | 0โ0 (a.e.t.) (4โ5 p) | New York Pancyprian-Freedoms (EPSL) | Highland Heights, Kentucky |
| 7:30 p.m. EDT | Report | Stadium: NKU Soccer Stadium Referee: Salvador Flores | ||
| Penalties | ||||
| March 20 | Tacoma Defiance (MLSNP) | 3โ1 | Washington Athletic Club (SRATS) | Tukwila, Washington |
| 7:00 p.m. PDT |
| Report |
| Stadium: Starfire Sports Complex Attendance: 205 Referee: Alex Wolbert |
| March 21 | Flatirons FC (USL2) | 1โ2 |  Union Omaha (USL1) | Golden, Colorado |
| 7:00 p.m. MDT |
| Report | Stadium: Stermole Soccer Stadium Attendance: 200 Referee: Lukasz Jozefecki |
Second round
| April 1 | Columbus Crew 2 (MLSNP) | 3โ0 | Lexington SC (USLC) | Columbus, Ohio |
| 6:00 p.m. EDT |
| Report | Stadium: Lower.com Field Attendance: 378 Referee: Salvador Flores |
| April 1 | South Georgia Tormenta FC (USL1) | 3โ2 | Foro SC (UPSL) | Statesboro, Georgia |
| 7:00 p.m. EDT |
| Report | Stadium: Tormenta Stadium Attendance: 467 Referee: Josiah Parke |
| April 1 | Inter Miami CF II (MLSNP) | 0โ1 | Miami FC (USLC) | Fort Lauderdale, Florida |
| 7:30 p.m. EDT | Report |
| Stadium: Chase Stadium Attendance: 1,500 Referee: Johnathan Bilinski |
| April 1 | Charlotte Independence (USL1) | 2โ1 (a.e.t.) | Carolina Core FC (MLSNP) | Matthews, North Carolina |
| 7:30 p.m. EDT | Report |
| Stadium: Sportsplex at Matthews Referee: John Rush |
| April 1 | Monterey Bay FC (USLC) | 1โ2 | El Farolito SC (NPSL) | Seaside, California |
| 7:00 p.m. PDT | Report |
| Stadium: Cardinale Stadium Attendance: 688 Referee: Esad Omanovic |
| April 1 | AV Alta FC (USL1) | 2โ1 (a.e.t.) | Los Angeles FC 2 (MLSNP) | Lancaster, California |
| 7:30 p.m. PDT |
| Report |
| Stadium: Lancaster Municipal Stadium Attendance: 2,735 Referee: Servando Berna Rico |
| April 2 | Loudoun United FC (USLC) | 4โ2 | Virginia Dream FC (VSSL) | Leesburg, Virginia |
| 6:30 p.m. EDT | Report |
| Stadium: Segra Field Referee: Marie Durr |
| April 2 | Portland Hearts of Pine (USL1) | 1โ1 (a.e.t.) (4โ2 p) | Hartford Athletic (USLC) | Lewiston, Maine |
| 6:30 p.m. EDT |
| Report |
| Stadium: Franklin Athletic Complex Attendance: 3,002 Referee: Kyle Averill |
| Penalties | ||||
| April 2 | Westchester SC (USL1) | 3โ2 | New York Pancyprian-Freedoms (EPSL) | Mount Vernon, New York |
| 7:00 p.m. EDT | Report |
| Stadium: The Stadium at Memorial Field Attendance: 2,026 |
| April 2 | Chattanooga FC (MLSNP) | 1โ1 (a.e.t.) (4โ5 p) | Chattanooga Red Wolves SC (USL1) | Chattanooga, Tennessee |
| 7:00 p.m. EDT |
| Report | Stadium: Finley Stadium Attendance: 12,131 Referee: Richonne Clark | |
| Penalties | ||||
| ||||
| April 2 | Greenville Triumph SC (USL1) | 1โ3 | One Knoxville SC (USL1) | Greenville, South Carolina |
| 7:00 p.m. EDT |
| Report |
| Stadium: Paladin Stadium Attendance: 854 Referee: Izlen Peksenar |
| April 2 | FC Naples (USL1) | 3โ0 | Little Rock Rangers (USL2) | Naples, Florida |
| 7:30 p.m. EDT | Report | Stadium: Paradise Coast Sports Complex Stadium Attendance: 2,174 Referee: Edson Caravajal |
| April 2 | Forward Madison FC (USL1) | 1โ3 | FC Tulsa (USLC) | Madison, Wisconsin |
| 6:30 p.m. CDT |
| Report | Stadium: Breese Stevens Field Attendance: 832 Referee: Felix Granados |
| April 2 | Des Moines Menace (USL2) | 1โ2 | Union Omaha (USL1) | West Des Moines, Iowa |
| 7:30 p.m. CDT | Report | Stadium: Valley Stadium Attendance: 1,000 Referee: Christian Campo Hernandez |
| April 2 | Tacoma Defiance (MLSNP) | 2โ1 | Spokane Velocity FC (USL1) | Tukwila, Washington |
| 7:00 p.m. PDT | Report |
| Stadium: Starfire Sports Complex Attendance: 2,300 Referee: Christopher Calderon |
| April 2 | Ventura County FC (MLSNP) | 0โ3 | El Paso Locomotive FC (USLC) | Thousand Oaks, California |
| 7:30 p.m. PDT | Report |
| Stadium: Dignity Health Sports Park Track & Field Stadium Attendance: 50 Referee: Steven Cardozo |
Third round
The complete draw for the third round, including match dates and times, was announced on April 3, 2025.
| April 15 | Columbus Crew 2 (MLSNP) | 0โ1 | Pittsburgh Riverhounds SC (USLC) | Columbus, Ohio |
| 6:00 p.m. EDT | Report |
| Stadium: Lower.com Field Attendance: 376 Referee: Rogelio Alvarez |
| April 15 | Louisville City FC (USLC) | 2โ1 | Loudoun United FC (USLC) | Louisville, Kentucky |
| 7:00 p.m. EDT | Report |
| Stadium: Lynn Family Stadium Attendance: 2,642 Referee: Elivis Osmanovic |
| April 15 | Charlotte Independence (USL1) | 1โ3 | North Carolina FC (USLC) | Matthews, North Carolina |
| 7:00 p.m. EDT |
| Report | Stadium: Sportsplex at Matthews Referee: Danielle Chesney |
| April 15 | Portland Hearts of Pine (USL1) | 1โ2 |  Rhode Island FC (USLC) | Lewiston, Maine |
| 7:00 p.m. EDT |
| Report |
| Stadium: Franklin Athletic Complex Attendance: 2,933 Referee: Thomas Snyder |
| April 15 | Charleston Battery (USLC) | 4โ0 | South Georgia Tormenta FC (USL1) | Mount Pleasant, South Carolina |
| 7:30 p.m. EDT | Report | Stadium: Ralph Lundy Field Attendance: 1,419 Referee: John Rush |
| April 15 | Union Omaha (USL1) | 1โ0 | San Antonio FC (USLC) | Omaha, Nebraska |
| 7:00 p.m. CDT |
| Report | Stadium: Caniglia Field Referee: Drew Klemp |
| April 15 | AV Alta FC (USL1) | 2โ2 (a.e.t.) (4โ2 p) | Orange County SC (USLC) | Lancaster, California |
| 7:00 p.m. PDT |
| Report |
| Stadium: Lancaster Municipal Stadium |
| Penalties | ||||
| April 15 | Tacoma Defiance (MLSNP) | 2โ1 (a.e.t.) | Oakland Roots SC (USLC) | Tukwila, Washington |
| 7:30 p.m. PDT |
| Report | Stadium: Starfire Sports Complex |
| April 16 | Detroit City FC (USLC)  | 3โ1 | Westchester SC (USL1) | Hamtramck, Michigan |
| 7:00 p.m. EDT | Report |
| Stadium: Keyworth Stadium Referee: Edson Carvajal |
| April 16 | FC Naples (USL1) | 1โ1 (a.e.t.) (8โ9 p) | Tampa Bay Rowdies (USLC) | Naples, Florida |
| 7:00 p.m. EDT |
| Report |
| Stadium: Paradise Coast Sports Complex Stadium Attendance: 3,418 Referee: Muhammad Hassan |
| Penalties | ||||
| ||||
| April 16 | Indy Eleven (USLC) | 1โ0 (a.e.t.) | Miami FC (USLC) | Indianapolis, Indiana |
| 7:30 p.m. EDT |
| Report | Stadium: Michael A. Carroll Stadium Attendance: 4,366 Referee: Calin Radosav |
| April 16 | FC Tulsa (USLC) | 1โ1 (a.e.t.) (2โ4 p) | Phoenix Rising FC (USLC) | Tulsa, Oklahoma |
| 7:00 p.m. CDT |
| Report |
| Stadium: Hurricane Soccer & Track Stadium Referee: Rodrigo Albuquerque |
| Penalties | ||||
| April 16 | Colorado Springs Switchbacks FC (USLC) | 3โ2 (a.e.t.) | One Knoxville SC (USL1) | Colorado Springs, Colorado |
| 7:00 p.m. MDT |
| Report |
| Stadium: Weidner Field Referee: Jay Schlesinger |
| April 16 | New Mexico United (USLC)  | 2โ2 (a.e.t.) (1โ4 p) | El Paso Locomotive (USLC) | Albuquerque, New Mexico |
| 7:30 p.m. MDT | Report |
| Stadium: UNM Soccer Complex Referee: John Griggs | |
| Penalties | ||||
| April 16 | Sacramento Republic FC (USLC) | 1โ0 | El Farolito SC (NPSL) | Sacramento, California |
| 7:00 p.m. PDT |
| Report | Stadium: Heart Health Park Referee: Robert Vincze |
| April 16 | Las Vegas Lights FC (USLC)  | 2โ2 (a.e.t.) (3โ4 p) | Chattanooga Red Wolves SC (USL1) | Las Vegas, Nevada |
| 7:30 p.m. PDT | Report |
| Stadium: Cashman Field Referee: Jaclyn Metz | |
| Penalties | ||||
Round of 32 and 16
The draw for the round of 32 and round of 16 took place live on CBS Sports Golazo Network on April 17, 2025, performed by CBS Sports Golazo Network presenter Aly Trost Martin and U.S. Open Cup commissioner David Applegate. The teams were divided into eight broadly geographical groups named for the eight most-capped United States men's national team players who had also won a U.S. Open Cup: Cobi Jones, Landon Donovan, Clint Dempsey, Jeff Agoos, DaMarcus Beasley, Carlos Bocanegra, Paul Caligiuri and Kasey Keller. The winners of four-team mini-bracket will advance to the quarterfinals. The order in which teams were drawn for the round of 32 determined the priority order for home field advantage in the round of 16.
Teams
- Bold denotes teams that are automatically seeded as hosts in the Round of 32.
Bracket
Bold denotes the winners of each match.
Host team listed first
Bold = winner
Round of 32
Cobi Jones Group
| May 7, 2025 | Rhode Island FC | 1โ2 | New England Revolution | Pawtucket, Rhode Island |
| 7:30 p.m. EDT | Rodriguez 50' |
| Stadium: Centreville Bank Stadium Attendance: 9,539 |
| May 7, 2025 | Chicago Fire FC  | 4โ0 | Detroit City FC | Bridgeview, Illinois |
| 7:00 p.m. CDT |
| Stadium: SeatGeek Stadium Attendance: 4,374 |
Landon Donovan Group
| May 7, 2025 | Pittsburgh Riverhounds SC | 1โ0 | New York City FC | Pittsburgh, Pennsylvania |
| 7:00 p.m. EDT | Ydrach90+7' | Stadium: Highmark Stadium Attendance: 3,661 |
| May 7, 2025 | Philadelphia Union | 1โ1 (a.e.t.) (5โ4 p) | Indy Eleven | Chester, Pennsylvania |
| 7:30 p.m. EDT | Bedoya6' | Amoh48' | Stadium: Subaru Park Attendance: 8,633 | |
| Penalties | ||||
Clint Dempsey Group
| May 6, 2025 | North Carolina FC | 1โ4 (a.e.t.) | Charlotte FC | Cary, North Carolina |
| 7:00 p.m. EDT | Mentzingen98' | Abada97' Agyemang 104'Petkoviฤ 119'Vargas 120+3' | Stadium: WakeMed Soccer Park Attendance: 4,155 |
| May 6, 2025 | D.C. United  | 2โ0 (a.e.t.) | Charleston Battery | Washington, D.C. |
| 7:00 p.m. EDT | Murrell96' Stroud 103' | Report | Stadium: Audi Field Attendance: 6,781 Referee: Ricardo Montero |
Jeff Agoos Group
| May 7, 2025 | Tampa Bay Rowdies | 0โ5 | Orlando City SC | St. Petersburg, Florida |
| 7:30 p.m. EDT |
| Stadium: Al Lang Stadium Attendance: 3,735 |
| May 6, 2025 | Nashville SC | 1โ0 | Chattanooga Red Wolves SC | Nashville, Tennessee |
| 7:00 p.m. CDT | Bauer18' | Stadium: Geodis Park Referee: Jeremy Scheer (Connecticut) |
DaMarcus Beasley Group
| May 7, 2025 | Louisville City FC | 0โ1 | Minnesota United FC | Louisville, Kentucky |
| 7:30 p.m. EDT |
| Stadium: Lynn Family Stadium Attendance: 4,482 |
| May 7, 2025 | St. Louis City SC | 2โ0 | Union Omaha | St. Louis, Missouri |
| 7:00 p.m. CDT | Stadium: Energizer Park |
Carlos Bocanegra Group
| May 7, 2025 | Phoenix Rising FC | 1โ4 (a.e.t.) | Houston Dynamo FC | Phoenix, Arizona |
| 7:00 p.m. MST | Stadium: Phoenix Rising Soccer Stadium Attendance: 4,346 |
| May 7, 2025 | Austin FC | 3โ2 | El Paso Locomotive FC | Austin, Texas |
| 7:30 p.m. CDT |
| Stadium: Q2 Stadium |
Paul Caligiuri Group
| May 6, 2025 | Colorado Springs Switchbacks FC | 1โ4 | New York Red Bulls | Colorado Springs, Colorado |
| 7:30 p.m. MDT |
| Stadium: Weidner Field |
| May 7, 2025 | FC Dallas | 3โ1 | AV Alta FC | Frisco, Texas |
| 7:00 p.m. CDT |
| Stadium: Toyota Stadium Attendance: 4,892 |
Kasey Keller Group
| May 6, 2025 | Tacoma Defiance | 2โ3 |  Portland Timbers | Tukwila, Washington |
| 7:00 p.m. PDT | De Rosario48' Tsukanome 50' | Report | McGraw33' Guerra 80'Kelsy 90+3' | Stadium: Starfire Sports Complex Attendance: 1,792 Referee: Servando Rico |
| May 7, 2025 | San Jose Earthquakes | 2โ1 | Sacramento Republic FC | San Jose, California |
| 7:30 p.m. PDT | Pellegrino2' Judd 44' | Amann90+6' | Stadium: PayPal Park |
Round of 16
| May 20, 2025 | New England Revolution | v | Chicago Fire FC | Providence, Rhode Island |
| 7:30 p.m. EDT | Stadium: Chapey Field at Anderson Stadium |
| May 21, 2025 | Philadelphia Union | v | Pittsburgh Riverhounds SC | Chester, Pennsylvania |
| 7:30 p.m. EDT | Stadium: Subaru Park |
| May 21, 2025 | D.C. United | v | Charlotte FC | Washington, D.C. |
| 7:30 p.m. EDT | Stadium: Audi Field |
| May 21, 2025 | Orlando City SC | v | Nashville SC | Orlando, Florida |
| 7:30 p.m. EDT | Stadium: Inter&Co Stadium |
| May 21, 2025 | Minnesota United FC | v | St. Louis City SC | St. Paul, Minnesota |
| 6:30 p.m. CDT | Stadium: Allianz Field |
| May 21, 2025 | Austin FC | v | Houston Dynamo FC | Austin, Texas |
| 8:00 p.m. CDT | Stadium: Q2 Stadium |
| May 21, 2025 | New York Red Bulls | v | FC Dallas | Harrison, New Jersey |
| 7:30 p.m. CDT | Stadium: Sports Illustrated Stadium |
| May 20, 2025 | San Jose Earthquakes | v | Portland Timbers | San Jose, California |
| 7:30 p.m. PDT | Stadium: PayPal Park |
Quarterfinals and beyond
Quarterfinals
Semifinals
Final
The 2025 U.S. Open Cup winner earns a place in the 2026 CONCACAF Champions Cup.
Top goal scorers
- As of May 7, 2025.
Team eliminated for this round.
Team was inactive for this round.
Broadcasting
TNT Sports holds the rights to broadcast the Open Cup from 2023 to 2030, as part of a larger media rights agreement with U.S. Soccer. The U.S. Soccer YouTube channel will broadcast all games from the first two rounds. On March 26, 2025, CBS Sports announced an agreement to exclusively broadcast all games beginning with the third round. All games will stream on Paramount+, with select games on the CBS Sports Golazo Network and CBS Sports Network.