Splicing factor 1

SF1
Identifiers
Aliases	SF1, BBP, D11S636, MBBP, ZCCHC25, ZFM1, ZNF162, splicing factor 1
External IDs	OMIM: 601516; MGI: 1095403; GeneCards: SF1
Available structures
PDB	Ortholog search: PDBe RCSB
List of PDB id codes
	1K1G, 1O0P, 1OPI, 2M09, 4FXW, 4FXX
Gene location (Human)
Chr.	Chromosome 11 (human)
End	64,778,786 bp
RNA expression pattern
	Top expressed in
	right uterine tube; ; left ovary; ; tibial nerve; ; gastric mucosa; ; right ovary; ; right lobe of thyroid gland; ; left uterine tube; ; canal of the cervix; ; right hemisphere of cerebellum; ; left lobe of thyroid gland;
	n/a
	More reference expression data
	More reference expression data
Gene ontology
Molecular function	transcription corepressor activity; zinc ion binding; metal ion binding; protein binding; nucleic acid binding; identical protein binding; pre-mRNA branch point binding; RNA binding;
Cellular component	ribosome; spliceosomal complex; nucleus; nucleoplasm; nuclear body;
Biological process	negative regulation of smooth muscle cell proliferation; regulation of transcription, DNA-templated; Leydig cell differentiation; mRNA processing; regulation of steroid biosynthetic process; male sex determination; transcription, DNA-templated; spliceosomal complex assembly; mRNA 3'-splice site recognition; RNA splicing; negative regulation of nucleic acid-templated transcription; mRNA splicing, via spliceosome; nuclear body organization;
	Sources:Amigo / QuickGO
Orthologs
Databases	NCBI: entry; OMA: entry
	7536
	22668
	ENSG00000168066
	ENSMUSG00000024949
	Q15637
	Q64213
NM_001178030; NM_001178031; NM_004630; NM_201995; NM_201997;
	NM_201998; NM_001346363; NM_001346364; NM_001346409; NM_001346410; NM_001378956; NM_001378957
	NM_001110791; NM_011750
NP_001171501; NP_001171502; NP_001333292; NP_001333293; NP_001333338;
	NP_001333339; NP_004621; NP_973724; NP_973726; NP_973727; NP_001365885; NP_001365886
NP_001104261; NP_035880; NP_001347322; NP_001390363; NP_001390364;
	NP_001390365; NP_001390368; NP_001390369; NP_001390370; NP_001390371; NP_001390372; NP_001390373; NP_001390374; NP_001390375; NP_001390376; NP_001390377; NP_001390378
	Wikidata
View/Edit Human	View/Edit Mouse

Splicing factor 1 is a protein that in humans is encoded by the SF1 gene (previously ZNF162). This protein is involved in removing introns from pre-mRNA as part of RNA splicing. In yeast, it is also frequently called Branchpoint Binding Protein (BBP), since it binds to the branch point sequence (BPS) of introns.

Function

SF1 is a splicing factor which contributes to the formation of the major spliceosome complex. In particular, after the U2AF1-U2AF2 heterodimer binds to the 3’ end of the intron, SF1 binds to an adjacent motif in the RNA called the branch point sequence (BPS). Afterwards U2 spliceosomal RNA displaces SF1, and itself binds to the BPS.

In yeast

The role of the protein in yeast cells has been the subject of study. The BPS that the protein binds to is UACUAAC.

Interactions

SF1 (gene) has been shown to interact with Ewing sarcoma breakpoint region 1, U2AF2, and transcription elongation regulator 1.

References

1 2 3 GRCh38: Ensembl release 89: ENSG00000168066 – Ensembl, May 2017
↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ Toda T, Iida A, Miwa T, Nakamura Y, Imai T (July 1994). "Isolation and characterization of a novel gene encoding nuclear protein at a locus (D11S636) tightly linked to multiple endocrine neoplasia type 1 (MEN1)". Human Molecular Genetics. 3 (3): 465–470. doi:10.1093/hmg/3.3.465. PMID 7912130.
↑ Kramer A, Quentin M, Mulhauser F (June 1998). "Diverse modes of alternative splicing of human splicing factor SF-1 deduced from the exon-intron structure of the gene". Gene. 211 (1): 29–37. doi:10.1016/S0378-1119(98)00058-4. PMID 9573336.
↑ "Entrez Gene: SF1 splicing factor 1".
1 2 Rino J, Desterro JM, Pacheco TR, Gadella TW, Carmo-Fonseca M (May 2008). "Splicing factors SF1 and U2AF associate in extraspliceosomal complexes". Molecular and Cellular Biology. 28 (9): 3045–3057. doi:10.1128/MCB.02015-07. PMC 2293075. PMID 18285458.
↑ "BBP Involved in Splice Site Recognition". Archived from the original on 2010-05-31.
↑ Niño CA, Scotto di Perrotolo R, Polo S (2022-01-07). "Recurrent spliceosome mutations in cancer: mechanisms and consequences of aberrant splice site selection". Cancers. 14 (2). Basel: 281. doi:10.3390/cancers14020281. PMC 8773931.
↑ Rymond BC (2010). The branchpoint binding protein: in and out of the spliceosome cycle. Adv Exp Med Biol. Vol. 693. pp. 123–141. doi:10.1007/978-1-4419-7005-3_9. PMID 21189690.
↑ Berglund JA, Chua K, Abovich N, Reed R, Rosbash M (May 1997). "The splicing factor BBP interacts specifically with the pre-mRNA branchpoint sequence UACUAAC". Cell. 89 (5): 781–787. doi:10.1016/S0092-8674(00)80261-5. PMID 9182766. S2CID 10505875.
↑ Zhang D, Paley AJ, Childs G (July 1998). "The transcriptional repressor ZFM1 interacts with and modulates the ability of EWS to activate transcription". The Journal of Biological Chemistry. 273 (29): 18086–18091. doi:10.1074/jbc.273.29.18086. PMID 9660765.
↑ Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, et al. (October 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–1178. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
↑ Berglund JA, Abovich N, Rosbash M (March 1998). "A cooperative interaction between U2AF65 and mBBP/SF1 facilitates branchpoint region recognition". Genes & Development. 12 (6): 858–867. doi:10.1101/gad.12.6.858. PMC 316625. PMID 9512519.
↑ Abovich N, Rosbash M (May 1997). "Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals". Cell. 89 (3): 403–412. doi:10.1016/S0092-8674(00)80221-4. PMID 9150140. S2CID 18466775.
↑ Goldstrohm AC, Albrecht TR, Suñé C, Bedford MT, Garcia-Blanco MA (November 2001). "The transcription elongation factor CA150 interacts with RNA polymerase II and the pre-mRNA splicing factor SF1". Molecular and Cellular Biology. 21 (22): 7617–7628. doi:10.1128/MCB.21.22.7617-7628.2001. PMC 99933. PMID 11604498.

Krämer A (October 1992). "Purification of splicing factor SF1, a heat-stable protein that functions in the assembly of a presplicing complex". Molecular and Cellular Biology. 12 (10): 4545–4552. doi:10.1128/MCB.12.10.4545. PMC 360381. PMID 1406644.
Naumovski L, Cleary ML (1996). "The p53-binding protein 53BP2 also interacts with Bc12 and impedes cell cycle progression at G2/M". Molecular and Cellular Biology. 16 (7): 3884–3892. doi:10.1128/MCB.16.7.3884. PMC 231385. PMID 8668206.
Arning S, Grüter P, Bilbe G, Krämer A (1996). "Mammalian splicing factor SF1 is encoded by variant cDNAs and binds to RNA". RNA. 2 (8). New York, N.Y.: 794–810. PMC 1369416. PMID 8752089.
Caslini C, Spinelli O, Cazzaniga G, Golay J, De Gioia L, Pedretti A, et al. (June 1997). "Identification of two novel isoforms of the ZNF162 gene: a growing family of signal transduction and activator of RNA proteins". Genomics. 42 (2): 268–277. doi:10.1006/geno.1997.4705. PMID 9192847.
Zhang D, Childs G (1998). "Human ZFM1 protein is a transcriptional repressor that interacts with the transcription activation domain of stage-specific activator protein". The Journal of Biological Chemistry. 273 (12): 6868–6877. doi:10.1074/jbc.273.12.6868. PMID 9506990.
Neubauer G, King A, Rappsilber J, Calvio C, Watson M, Ajuh P, et al. (September 1998). "Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex". Nature Genetics. 20 (1): 46–50. doi:10.1038/1700. PMID 9731529. S2CID 585778.
Wang X, Bruderer S, Rafi Z, Xue J, Milburn PJ, Krämer A, et al. (August 1999). "Phosphorylation of splicing factor SF1 on Ser20 by cGMP-dependent protein kinase regulates spliceosome assembly". The EMBO Journal. 18 (16): 4549–4559. doi:10.1093/emboj/18.16.4549. PMC 1171529. PMID 10449420.
Lapteva N, Nieda M, Ando Y, Ide K, Hatta-Ohashi Y, Dymshits G, et al. (July 2001). "Expression of renin-angiotensin system genes in immature and mature dendritic cells identified using human cDNA microarray". Biochemical and Biophysical Research Communications. 285 (4): 1059–1065. doi:10.1006/bbrc.2001.5215. PMID 11467860.
Liu Z, Luyten I, Bottomley MJ, Messias AC, Houngninou-Molango S, Sprangers R, et al. (November 2001). "Structural basis for recognition of the intron branch site RNA by splicing factor 1". Science. 294 (5544). New York, N.Y.: 1098–1102. Bibcode:2001Sci...294.1098L. doi:10.1126/science.1064719. PMID 11691992. S2CID 21402023.
Rappsilber J, Ryder U, Lamond AI, Mann M (2002). "Large-scale proteomic analysis of the human spliceosome". Genome Research. 12 (8): 1231–1245. doi:10.1101/gr.473902. PMC 186633. PMID 12176931.
Selenko P, Gregorovic G, Sprangers R, Stier G, Rhani Z, Krämer A, et al. (April 2003). "Structural basis for the molecular recognition between human splicing factors U2AF65 and SF1/mBBP". Molecular Cell. 11 (4): 965–976. doi:10.1016/S1097-2765(03)00115-1. PMID 12718882.
Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, et al. (August 2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the United States of America. 101 (33): 12130–12135. Bibcode:2004PNAS..10112130B. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
Lin KT, Lu RM, Tarn WY (2004). "The WW domain-containing proteins interact with the early spliceosome and participate in pre-mRNA splicing in vivo". Molecular and Cellular Biology. 24 (20): 9176–9185. doi:10.1128/MCB.24.20.9176-9185.2004. PMC 517884. PMID 15456888.

This article on a gene on human chromosome 11 is a stub. You can help Wikipedia by adding missing information.

[refGRCh38Ensembl-1] 1 2 3 GRCh38: Ensembl release 89: ENSG00000168066 – Ensembl, May 2017

[2] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[3] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[Toda_1994-4] Toda T, Iida A, Miwa T, Nakamura Y, Imai T (July 1994). "Isolation and characterization of a novel gene encoding nuclear protein at a locus (D11S636) tightly linked to multiple endocrine neoplasia type 1 (MEN1)". Human Molecular Genetics. 3 (3): 465–470. doi:10.1093/hmg/3.3.465. PMID 7912130.

[Kramer_1998-5] Kramer A, Quentin M, Mulhauser F (June 1998). "Diverse modes of alternative splicing of human splicing factor SF-1 deduced from the exon-intron structure of the gene". Gene. 211 (1): 29–37. doi:10.1016/S0378-1119(98)00058-4. PMID 9573336.

[entrez-6] "Entrez Gene: SF1 splicing factor 1".

[Rino_2008-7] 1 2 Rino J, Desterro JM, Pacheco TR, Gadella TW, Carmo-Fonseca M (May 2008). "Splicing factors SF1 and U2AF associate in extraspliceosomal complexes". Molecular and Cellular Biology. 28 (9): 3045–3057. doi:10.1128/MCB.02015-07. PMC 2293075. PMID 18285458.

[8] "BBP Involved in Splice Site Recognition". Archived from the original on 2010-05-31.

[9] Niño CA, Scotto di Perrotolo R, Polo S (2022-01-07). "Recurrent spliceosome mutations in cancer: mechanisms and consequences of aberrant splice site selection". Cancers. 14 (2). Basel: 281. doi:10.3390/cancers14020281. PMC 8773931.

[Rymond2010-10] Rymond BC (2010). The branchpoint binding protein: in and out of the spliceosome cycle. Adv Exp Med Biol. Vol. 693. pp. 123–141. doi:10.1007/978-1-4419-7005-3_9. PMID 21189690.

[11] Berglund JA, Chua K, Abovich N, Reed R, Rosbash M (May 1997). "The splicing factor BBP interacts specifically with the pre-mRNA branchpoint sequence UACUAAC". Cell. 89 (5): 781–787. doi:10.1016/S0092-8674(00)80261-5. PMID 9182766. S2CID 10505875.

[Zhang_1998-12] Zhang D, Paley AJ, Childs G (July 1998). "The transcriptional repressor ZFM1 interacts with and modulates the ability of EWS to activate transcription". The Journal of Biological Chemistry. 273 (29): 18086–18091. doi:10.1074/jbc.273.29.18086. PMID 9660765.

[Rual_2005-13] Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, et al. (October 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–1178. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.

[Berglund_1998-14] Berglund JA, Abovich N, Rosbash M (March 1998). "A cooperative interaction between U2AF65 and mBBP/SF1 facilitates branchpoint region recognition". Genes & Development. 12 (6): 858–867. doi:10.1101/gad.12.6.858. PMC 316625. PMID 9512519.

[Abovich_1997-15] Abovich N, Rosbash M (May 1997). "Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals". Cell. 89 (3): 403–412. doi:10.1016/S0092-8674(00)80221-4. PMID 9150140. S2CID 18466775.

[Goldstrohm_2001-16] Goldstrohm AC, Albrecht TR, Suñé C, Bedford MT, Garcia-Blanco MA (November 2001). "The transcription elongation factor CA150 interacts with RNA polymerase II and the pre-mRNA splicing factor SF1". Molecular and Cellular Biology. 21 (22): 7617–7628. doi:10.1128/MCB.21.22.7617-7628.2001. PMC 99933. PMID 11604498.

Splicing factor 1

Function

In yeast

Interactions

References

Further reading