Vesicle-associated membrane protein 8

VAMP8
Identifiers
Aliases	VAMP8, EDB, VAMP-8, vesicle associated membrane protein 8
External IDs	OMIM: 603177; MGI: 1336882; GeneCards: VAMP8
Available structures
PDB	Ortholog search: PDBe RCSB
List of PDB id codes
	4WY4
Gene location (Human)
Chr.	Chromosome 2 (human)
End	85,582,031 bp
Gene location (Mouse)
Chr.	Chromosome 6 (mouse)
End	72,367,686 bp
RNA expression pattern
	Top expressed in
	palpebral conjunctiva; ; monocyte; ; mucosa of transverse colon; ; olfactory zone of nasal mucosa; ; granulocyte; ; jejunal mucosa; ; rectum; ; nasal epithelium; ; bronchial epithelial cell; ; skin of abdomen;
	Top expressed in
	proximal tubule; ; right kidney; ; yolk sac; ; granulocyte; ; lung; ; bone marrow; ; human kidney; ; urinary bladder; ; duodenum; ; lip;
	More reference expression data
	More reference expression data
Gene ontology
Molecular function	SNAP receptor activity; protein binding; chloride channel inhibitor activity; SNARE binding;
Cellular component	cytoplasm; integral component of membrane; recycling endosome; vesicle; cytosol; endosome; early endosome membrane; membrane; secretory granule membrane; late endosome membrane; plasma membrane; mucin granule; lysosomal membrane; SNARE complex; early endosome; perinuclear region of cytoplasm; lysosome; extracellular exosome; clathrin-coated vesicle membrane; phagocytic vesicle membrane; azurophil granule membrane; specific granule membrane; recycling endosome membrane; tertiary granule membrane; synaptic vesicle;
Biological process	post-Golgi vesicle-mediated transport; autophagy; autophagosome maturation; negative regulation of secretion by cell; protein transport; viral entry into host cell; vesicle fusion; eosinophil degranulation; vesicle-mediated transport; mucus secretion; regulation of protein localization to plasma membrane; positive regulation of histamine secretion by mast cell; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; autophagosome membrane docking; neutrophil degranulation; membrane organization; regulation of molecular function; transport;
	Sources:Amigo / QuickGO
Orthologs
Databases	NCBI: entry; OMA: entry
	8673
	22320
	ENSG00000118640
	ENSMUSG00000050732
	Q9BV40
	O70404
	NM_003761
	NM_016794
	NP_003752
	NP_058074
	Wikidata
View/Edit Human	View/Edit Mouse

Vesicle-associated membrane protein 8 is a protein that in humans is encoded by the VAMP8 gene.

Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. It is associated with the perinuclear vesicular structures of the early endocytic compartment. It has been found that VAMP8 interacts specifically with the soluble NSF-attachment protein (alpha-SNAP), most likely through an VAMP8-containing SNARE complex. Phosphorylation of VAMP8 inside the conserved SNARE-domain can suppress vesicle fusion.

In pancreatic β-cells, VAMP8 has been shown to be part of the endosomal system. It is particularly localized to Rab11 recycling endosomes, where it plays an important role in GLP1R and GLUT2 recycling. Overexpression of VAMP8 in β-cells results in decreased insulin secretion.

Interactions

Vesicle-associated membrane protein 8 has been shown to interact with STX4, SNAP23, STX1A, STX8 and STX7.

References

1 2 3 GRCh38: Ensembl release 89: ENSG00000118640 – Ensembl, May 2017
1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000050732 – Ensembl, May 2017
↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ Bui TD, Wong SH, Lu L, Hong W (February 1999). "Endobrevin maps to chromosome 2 in human and chromosome 6 in mouse". Genomics. 54 (3): 579–580. doi:10.1006/geno.1998.5596. PMID 9878266.
↑ Wong SH, Zhang T, Xu Y, Subramaniam VN, Griffiths G, Hong W (July 1998). "Endobrevin, a Novel Synaptobrevin/VAMP-Like Protein Preferentially Associated with the Early Endosome". Molecular Biology of the Cell. 9 (6): 1549–1563. doi:10.1091/mbc.9.6.1549. PMC 25382. PMID 9614193.
1 2 "Entrez Gene: VAMP8 vesicle-associated membrane protein 8 (endobrevin)".
↑ Malmersjö S, Di Palma S, Diao J, Lai Y, Pfuetzner RA, Wang AL, et al. (July 2016). "Phosphorylation of residues inside the SNARE complex suppresses secretory vesicle fusion". The EMBO Journal. 35 (16): 1721–1843. doi:10.15252/embj.201694071. PMC 5010044. PMID 27402227.
↑ Liu L, Marshall M, Chadeuf E, Saras J, Barg S (September 2025). "VAMP8 Is an Endosomal v-SNARE That Supports GLP-1 Receptor Recycling in Pancreatic β-Cells". Diabetes. 74 (9): 1577–1588. doi:10.2337/db24-0921. PMC 12365419. PMID 40608311.
1 2 Paumet F, Le Mao J, Martin S, Galli T, David B, Blank U, et al. (June 2000). "Soluble NSF attachment protein receptors (SNAREs) in RBL-2H3 mast cells: functional role of syntaxin 4 in exocytosis and identification of a vesicle-associated membrane protein 8-containing secretory compartment". Journal of Immunology. 164 (11). Baltimore: 5850–5857. doi:10.4049/jimmunol.164.11.5850. PMID 10820264.
↑ Polgár J, Chung SH, Reed GL (August 2002). "Vesicle-associated membrane protein 3 (VAMP-3) and VAMP-8 are present in human platelets and are required for granule secretion". Blood. 100 (3): 1081–1083. doi:10.1182/blood.V100.3.1081. PMID 12130530. S2CID 36597939.
↑ Imai A, Nashida T, Yoshie S, Shimomura H (August 2003). "Intracellular localisation of SNARE proteins in rat parotid acinar cells: SNARE complexes on the apical plasma membrane". Archives of Oral Biology. 48 (8): 597–604. doi:10.1016/S0003-9969(03)00116-X. PMID 12828989.
↑ Nagamatsu S, Nakamichi Y, Watanabe T, Matsushima S, Yamaguchi S, Ni J, et al. (January 2001). "Localization of cellubrevin-related peptide, endobrevin, in the early endosome in pancreatic beta cells and its physiological function in exo-endocytosis of secretory granules". Journal of Cell Science. 114 (Pt 1): 219–227. doi:10.1242/jcs.114.1.219. PMID 11112705.
1 2 Antonin W, Holroyd C, Fasshauer D, Pabst S, Von Mollard GF, Jahn R (December 2000). "A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function". The EMBO Journal. 19 (23): 6453–6464. doi:10.1093/emboj/19.23.6453. PMC 305878. PMID 11101518.
↑ Wade N, Bryant NJ, Connolly LM, Simpson RJ, Luzio JP, Piper RC, et al. (June 2001). "Syntaxin 7 complexes with mouse Vps10p tail interactor 1b, syntaxin 6, vesicle-associated membrane protein (VAMP)8, and VAMP7 in b16 melanoma cells". The Journal of Biological Chemistry. 276 (23): 19820–19827. doi:10.1074/jbc.M010838200. PMID 11278762.

Bonaldo MF, Lennon G, Soares MB (1997). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Research. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
Fasshauer D, Antonin W, Margittai M, Pabst S, Jahn R (May 1999). "Mixed and non-cognate SNARE complexes. Characterization of assembly and biophysical properties". The Journal of Biological Chemistry. 274 (22): 15440–15446. doi:10.1074/jbc.274.22.15440. PMID 10336434.
Kim PK, Hollerbach C, Trimble WS, Leber B, Andrews DW (December 1999). "Identification of the endoplasmic reticulum targeting signal in vesicle-associated membrane proteins". The Journal of Biological Chemistry. 274 (52): 36876–36882. doi:10.1074/jbc.274.52.36876. PMID 10601239.
Antonin W, Holroyd C, Tikkanen R, Höning S, Jahn R (October 2000). "The R-SNARE endobrevin/VAMP-8 mediates homotypic fusion of early endosomes and late endosomes". Molecular Biology of the Cell. 11 (10): 3289–3298. doi:10.1091/mbc.11.10.3289. PMC 14992. PMID 11029036.
Antonin W, Fasshauer D, Becker S, Jahn R, Schneider TR (February 2002). "Crystal structure of the endosomal SNARE complex reveals common structural principles of all SNAREs". Nature Structural Biology. 9 (2): 107–111. doi:10.1038/nsb746. hdl:11858/00-001M-0000-0012-F459-F. PMID 11786915. S2CID 17724790.
Hillier LW, Graves TA, Fulton RS, Fulton LA, Pepin KH, Minx P, et al. (April 2005). "Generation and annotation of the DNA sequences of human chromosomes 2 and 4". Nature. 434 (7034): 724–731. Bibcode:2005Natur.434..724H. doi:10.1038/nature03466. PMID 15815621.
Brinkman JF, Ottenheim CP, de Jong LA, Zegers RH, de Smet MD, de Jong PT, et al. (August 2005). "VAMP5 and VAMP8 are most likely not involved in primary open-angle glaucoma". Molecular Vision. 11: 582–586. PMID 16110299.
Oishi Y, Arakawa T, Tanimura A, Itakura M, Takahashi M, Tajima Y, et al. (March 2006). "Role of VAMP-2, VAMP-7, and VAMP-8 in constitutive exocytosis from HSY cells". Histochemistry and Cell Biology. 125 (3): 273–281. doi:10.1007/s00418-005-0068-y. PMID 16195891. S2CID 10541252.
Shiffman D, Rowland CM, Louie JZ, Luke MM, Bare LA, Bolonick JI, et al. (July 2006). "Gene variants of VAMP8 and HNRPUL1 are associated with early-onset myocardial infarction". Arteriosclerosis, Thrombosis, and Vascular Biology. 26 (7): 1613–1618. doi:10.1161/01.ATV.0000226543.77214.e4. PMID 16690874.

[refGRCh38Ensembl-1] 1 2 3 GRCh38: Ensembl release 89: ENSG00000118640 – Ensembl, May 2017

[refGRCm38Ensembl-2] 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000050732 – Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[Bui_1999-5] Bui TD, Wong SH, Lu L, Hong W (February 1999). "Endobrevin maps to chromosome 2 in human and chromosome 6 in mouse". Genomics. 54 (3): 579–580. doi:10.1006/geno.1998.5596. PMID 9878266.

[Wong_1998-6] Wong SH, Zhang T, Xu Y, Subramaniam VN, Griffiths G, Hong W (July 1998). "Endobrevin, a Novel Synaptobrevin/VAMP-Like Protein Preferentially Associated with the Early Endosome". Molecular Biology of the Cell. 9 (6): 1549–1563. doi:10.1091/mbc.9.6.1549. PMC 25382. PMID 9614193.

[entrez-7] 1 2 "Entrez Gene: VAMP8 vesicle-associated membrane protein 8 (endobrevin)".

[Malmersjö_2016-8] Malmersjö S, Di Palma S, Diao J, Lai Y, Pfuetzner RA, Wang AL, et al. (July 2016). "Phosphorylation of residues inside the SNARE complex suppresses secretory vesicle fusion". The EMBO Journal. 35 (16): 1721–1843. doi:10.15252/embj.201694071. PMC 5010044. PMID 27402227.

[9] Liu L, Marshall M, Chadeuf E, Saras J, Barg S (September 2025). "VAMP8 Is an Endosomal v-SNARE That Supports GLP-1 Receptor Recycling in Pancreatic β-Cells". Diabetes. 74 (9): 1577–1588. doi:10.2337/db24-0921. PMC 12365419. PMID 40608311.

[Paumet_2000-10] 1 2 Paumet F, Le Mao J, Martin S, Galli T, David B, Blank U, et al. (June 2000). "Soluble NSF attachment protein receptors (SNAREs) in RBL-2H3 mast cells: functional role of syntaxin 4 in exocytosis and identification of a vesicle-associated membrane protein 8-containing secretory compartment". Journal of Immunology. 164 (11). Baltimore: 5850–5857. doi:10.4049/jimmunol.164.11.5850. PMID 10820264.

[Polgár_2002-11] Polgár J, Chung SH, Reed GL (August 2002). "Vesicle-associated membrane protein 3 (VAMP-3) and VAMP-8 are present in human platelets and are required for granule secretion". Blood. 100 (3): 1081–1083. doi:10.1182/blood.V100.3.1081. PMID 12130530. S2CID 36597939.

[Imai_2003-12] Imai A, Nashida T, Yoshie S, Shimomura H (August 2003). "Intracellular localisation of SNARE proteins in rat parotid acinar cells: SNARE complexes on the apical plasma membrane". Archives of Oral Biology. 48 (8): 597–604. doi:10.1016/S0003-9969(03)00116-X. PMID 12828989.

[Nagamatsu_2001-13] Nagamatsu S, Nakamichi Y, Watanabe T, Matsushima S, Yamaguchi S, Ni J, et al. (January 2001). "Localization of cellubrevin-related peptide, endobrevin, in the early endosome in pancreatic beta cells and its physiological function in exo-endocytosis of secretory granules". Journal of Cell Science. 114 (Pt 1): 219–227. doi:10.1242/jcs.114.1.219. PMID 11112705.

[Antonin_2000-14] 1 2 Antonin W, Holroyd C, Fasshauer D, Pabst S, Von Mollard GF, Jahn R (December 2000). "A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function". The EMBO Journal. 19 (23): 6453–6464. doi:10.1093/emboj/19.23.6453. PMC 305878. PMID 11101518.

[Wade_2001-15] Wade N, Bryant NJ, Connolly LM, Simpson RJ, Luzio JP, Piper RC, et al. (June 2001). "Syntaxin 7 complexes with mouse Vps10p tail interactor 1b, syntaxin 6, vesicle-associated membrane protein (VAMP)8, and VAMP7 in b16 melanoma cells". The Journal of Biological Chemistry. 276 (23): 19820–19827. doi:10.1074/jbc.M010838200. PMID 11278762.

Vesicle-associated membrane protein 8

Interactions

References

Further reading