LaCrosse is a town in Alachua County, in the U.S. state of Florida. The 2020 United States Census counted 316 people living in the town.

LaCrosse developed as a farming community during the 1800s. The town was incorporated on December 17, 1897. Cotton, corn and other crops were important to the early community.
The town is in northern Alachua County. Farming remains an important part of the surrounding area.