Absolute genetic divergence

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Absolute genetic divergence, often denoted as ${\displaystyle d_{XY}}$, is a measure used in population genetics to quantify the average number of nucleotide differences per site between two DNA sequences drawn from different populations. Unlike relative measures of divergence such as the Fixation index (${\displaystyle F_{ST}}$), absolute genetic divergence is independent of the genetic diversity within the populations being compared. It is frequently used in evolutionary biology to detect gene flow, estimate species divergence times, and investigate "genomic islands" of speciation.

Definition and Calculation

Absolute genetic divergence was formally defined by Masatoshi Nei in 1987. It represents the average number of pairwise nucleotide differences between all possible pairs of sequences where one is taken from population X and the other from population Y.

Mathematically, it is calculated as:

${\displaystyle d_{XY}=\sum _{i,j}x_{i}y_{j}d_{ij}}$

Where:

• ${\displaystyle x_{i}}$ is the frequency of the i-th haplotype in population X.
• ${\displaystyle y_{j}}$ is the frequency of the j-th haplotype in population Y.
• ${\displaystyle d_{ij}}$ is the number of nucleotide differences between haplotype i and haplotype j.

Because it measures the average pairwise differences regardless of population subdivision, ${\displaystyle d_{XY}}$ is mathematically equivalent to the total nucleotide diversity (${\displaystyle \pi _{Total}}$) if the two populations were pooled together.

Comparison with Relative Divergence (${\displaystyle F_{ST}}$)

A major application of ${\displaystyle d_{XY}}$ is to distinguish between evolutionary processes that affect genetic variation within populations versus those that affect divergence between populations.

• Relative Divergence (${\displaystyle F_{ST}}$): Measures population differentiation relative to the total genetic variance. It can be inflated solely by a reduction in within-population diversity (${\displaystyle \pi }$), even if the populations have not actually diverged significantly in terms of sequence mutations.
• Absolute Divergence (${\displaystyle d_{XY}}$): Measures the accumulation of sequence differences. It is generally not affected by current within-population diversity or selective sweeps that reduce local variation.

In the study of "genomic islands of speciation" (regions of the genome with high differentiation), ${\displaystyle d_{XY}}$ is often used as a control statistic. If a genomic region has high ${\displaystyle F_{ST}}$ but normal or low ${\displaystyle d_{XY}}$, the differentiation is likely driven by reduced diversity (e.g., linked selection or background selection) rather than accelerated divergence or a barrier to gene flow.

Relation to Ancestral Diversity

Absolute divergence is influenced by the diversity of the ancestral population. The value of ${\displaystyle d_{XY}}$ reflects both the mutations accumulated after the populations split and the polymorphism that was present in the common ancestor.

${\displaystyle E(d_{XY})=2\mu t+\theta _{Anc}}$

Where:

• ${\displaystyle \mu }$ is the mutation rate.
• ${\displaystyle t}$ is the time since divergence.
• ${\displaystyle \theta _{Anc}}$ is the ancestral nucleotide diversity.

Because of this relationship, ${\displaystyle d_{XY}}$ requires a significant amount of time to accumulate after speciation, leading to a "time lag" in its utility for detecting very recent divergence compared to other metrics.

See also

• Fixation index (${\displaystyle F_{ST}}$)
• Nucleotide diversity
• Genetic distance
• Coalescent theory

References