Alcian yellow

Alcian yellow
Names
IUPAC name
{(E)-1,2-Diazenediylbis[4,1-phenylene(6-methyl-1,3-benzothiazole-2,5-diyl)methylenesulfanediyl]}bis[(dimethylamino)-N,N-dimethylmethaniminium]
Other names
Ingrain Yellow 1
Identifiers
3D model (JSmol)
ChEBI
ChemSpider
ECHA InfoCard 100.115.894 Edit this at Wikidata
  • InChI=1S/C40H46N8S4/c1-25-19-35-33(21-29(25)23-49-39(45(3)4)46(5)6)41-37(51-35)27-11-15-31(16-12-27)43-44-32-17-13-28(14-18-32)38-42-34-22-30(26(2)20-36(34)52-38)24-50-40(47(7)8)48(9)10/h11-22H,23-24H2,1-10H3/q+2/b44-43+
    Key: QMKQNEXRGZFFKQ-VGFSZAGXSA-N
  • InChI=1/C40H46N8S4/c1-25-19-35-33(21-29(25)23-49-39(45(3)4)46(5)6)41-37(51-35)27-11-15-31(16-12-27)43-44-32-17-13-28(14-18-32)38-42-34-22-30(26(2)20-36(34)52-38)24-50-40(47(7)8)48(9)10/h11-22H,23-24H2,1-10H3/q+2/b44-43+
    Key: QMKQNEXRGZFFKQ-VGFSZAGXBQ
  • Cc1cc2c(cc1CSC(=[N+](C)C)N(C)C)nc(s2)c3ccc(cc3)/N=N/c4ccc(cc4)c5nc6cc(c(cc6s5)C)CSC(=[N+](C)C)N(C)C
Properties
C40H46N8S4
Molar mass 767.11 g/mol
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).

Alcian yellow is an azo dye with the formula (CH3[(CH3N)2CS]2C6H2SNCC6H4)2N2. Its structure consists of a largely conjugated array of benzene, benzothiazole and isothiouronium units.

Histological Use

Alcian yellow is used in histology in conjunction with toluidine blue to help visualize H. pylori. The resulting structures are stained as follows:

– Mucin: yellow

– H. pylori: blue

– Other cellular components/background: shades of blue

See also

References

  1. ↑ Leung, Jennifer K.; Gibbon, Kevin J.; Vartanian, Robert K. (1996-06-01). "Rapid Staining Method for Helicobacter Pylori in Gastric Biopsies". Journal of Histotechnology. 19 (2): 131–132. doi:10.1179/his.1996.19.2.131. ISSN 0147-8885.
  2. ↑ "Helico*Stat Rapid Helicobacter Stain Kit Procedure" (PDF). Archived from the original (PDF) on 2017-03-06. Retrieved 2017-03-05.