Carnoy's solution

Carnoy's solution is a fixative composed of 60% ethanol, 30% chloroform and 10% glacial acetic acid, 1 gram of ferric chloride.

Carnoy's solution is also the name of a different fixation composed of ethanol and glacial acetic acid (3:1).

The invention of Carnoy's solution is attributed to Jean-Baptiste Carnoy, a pioneering 19th century cytologist.

Uses

Some of the uses of Carnoy's solution are:

Enhancing lymph node detection during dissection of cadavers.
Immunohistochemical fixation and detection of NMDA receptors within the murine hippocampus.
Applied directly following enucleation for the treatment of odontogenic keratocysts.
Direct application following enucleation (Cuba) for certain kinds of unicystic ameloblastomas. This appears to decrease the likelihood of recurrence over enucleation alone. Protein coagulation is thought to limit uptake of these toxic materials by surrounding tissues, however it is this fact that limits its usefulness as a treatment agent in general.
As a fixative for pap smear samples.
As a fixative agent for both nuclear and mitochondrial DNA in various tissues.
As a fixative agent to preserve mucus, useful for tissue preparation before staining with periodic acid-Schiff base.

References

↑ "MSDS :: Carnoy's Solution (Fixative)". Archived from the original on 2009-03-27. Retrieved 13 Jan 2009.
↑ Carnoy J. B. (1887). "Appendice Les Globule Polaires de L'Ascaris Clavata". La Cellule RECUEIL DE CYTOLOGIE ET d'HISTOLOGIE GÉNÉRALE. 3: 276.
↑ Tjio JH, Whang J (1962). "Chromosome Preparatons of Bone Marrow Cells without PriorIn VitroCulture orIn VivoColchicine Administration". Stain Technology. 37: 17–20. doi:10.3109/10520296209114563. PMID 13921436.
↑ Mazia D, Brewer PA, Alfert MA (1953). "The Cytochemical Staining and Measurement of Protein with Mercuric Bromphenol Blue". The Biological Bulletin. 104 (1): 57–67. doi:10.2307/1538691. JSTOR 1538691.
↑ Utevsky S, Kovalenko N, Doroshenko K, Petrauskienė L, Klymenko V (2009). "Chromosome numbers for three species of medicinal leeches (Hirudo spp.)". Systematic Parasitology. 74 (2): 95–102. doi:10.1007/s11230-009-9198-2. PMID 19731093. S2CID 7947757.
↑ Ireland, Robert; Yeung, Chuen Albert YeungChuen Albert (2020-04-23), "Carnoy's solution", in Yeung, Chuen Albert (ed.), A Dictionary of Dentistry, Oxford University Press, doi:10.1093/acref/9780191828621.001.0001, ISBN 978-0-19-182862-1, retrieved 2023-04-10
↑ Luz DA, Ribeiro U, Chassot C, Collet E, Silva FS, Cecconello I, Corbett CE (December 2008). "Carnoy's solution enhances lymph node detection: an anatomical dissection study in cadavers". Histopathology. 53 (6): 740–2. doi:10.1111/j.1365-2559.2008.03148.x. PMID 19076686. S2CID 19362456.
↑ Yoneyama M, Kitayama T, Taniura H, Yoneda Y (August 2003). "Immersion fixation with Carnoy solution for conventional immunohistochemical detection of particular N-methyl-D-aspartate receptor subunits in murine hippocampus". J. Neurosci. Res. 73 (3): 416–26. doi:10.1002/jnr.10622. PMID 12868075. S2CID 40884078.
↑ Madras J, Lapointe H (March 2008). "Keratocystic odontogenic tumour: reclassification of the odontogenic keratocyst from cyst to tumour". J Can Dent Assoc. 74 (2): 165–165h. PMID 18353202.
↑ "Odontogenic Keratocyst: The Northwestern USA Experience". Archived from the original on 2009-01-06. Retrieved 14 Jan 2009.
↑ "Use of Carnoy's Solution in management of odontogenic keratocysts". Archived from the original on 2009-05-17. Retrieved 14 Jan 2009.
↑ Lee PK, Samman N, Ng IO (April 2004). "Unicystic ameloblastoma--use of Carnoy's solution after enucleation". Int J Oral Maxillofac Surg. 33 (3): 263–7. doi:10.1006/ijom.2003.0496. PMID 15290793.
↑ Lau SL, Samman N (August 2006). "Recurrence related to treatment modalities of unicystic ameloblastoma: a systematic review". Int J Oral Maxillofac Surg. 35 (8): 681–90. doi:10.1016/j.ijom.2006.02.016. PMID 16782308.
↑ Marx, Robert E.; Stern, Diane (2003). Oral and maxillofacial pathology: a rationale for diagnosis and treatment. Chicago: Quintessence. p. 684. ISBN 0-86715-390-3.
↑ Shamsi M, Abdali K, Montazer NR, Kumar PV, Tabatabaee HR (2008). "Comparison of Carnoy's solution and 96% ethyl alcohol fixation in bloody Pap smears". Acta Cytol. 52 (2): 187–90. doi:10.1159/000325477. PMID 18499991. S2CID 3370408.
↑ Miething F, Hering S, Hanschke B, Dressler J (March 2006). "Effect of fixation to the degradation of nuclear and mitochondrial DNA in different tissues". J. Histochem. Cytochem. 54 (3): 371–4. doi:10.1369/jhc.5B6726.2005. PMID 16260588.
↑ "Stains File: Carnoy's Fluid". Archived from the original on 15 December 2010. Retrieved 25 Oct 2009.

[1] "MSDS :: Carnoy's Solution (Fixative)". Archived from the original on 2009-03-27. Retrieved 13 Jan 2009.

[2] Carnoy J. B. (1887). "Appendice Les Globule Polaires de L'Ascaris Clavata". La Cellule RECUEIL DE CYTOLOGIE ET d'HISTOLOGIE GÉNÉRALE. 3: 276.

[3] Tjio JH, Whang J (1962). "Chromosome Preparatons of Bone Marrow Cells without PriorIn VitroCulture orIn VivoColchicine Administration". Stain Technology. 37: 17–20. doi:10.3109/10520296209114563. PMID 13921436.

[4] Mazia D, Brewer PA, Alfert MA (1953). "The Cytochemical Staining and Measurement of Protein with Mercuric Bromphenol Blue". The Biological Bulletin. 104 (1): 57–67. doi:10.2307/1538691. JSTOR 1538691.

[5] Utevsky S, Kovalenko N, Doroshenko K, Petrauskienė L, Klymenko V (2009). "Chromosome numbers for three species of medicinal leeches (Hirudo spp.)". Systematic Parasitology. 74 (2): 95–102. doi:10.1007/s11230-009-9198-2. PMID 19731093. S2CID 7947757.

[6] Ireland, Robert; Yeung, Chuen Albert YeungChuen Albert (2020-04-23), "Carnoy's solution", in Yeung, Chuen Albert (ed.), A Dictionary of Dentistry, Oxford University Press, doi:10.1093/acref/9780191828621.001.0001, ISBN 978-0-19-182862-1, retrieved 2023-04-10

[pmid19076686-7] Luz DA, Ribeiro U, Chassot C, Collet E, Silva FS, Cecconello I, Corbett CE (December 2008). "Carnoy's solution enhances lymph node detection: an anatomical dissection study in cadavers". Histopathology. 53 (6): 740–2. doi:10.1111/j.1365-2559.2008.03148.x. PMID 19076686. S2CID 19362456.

[pmid12868075-8] Yoneyama M, Kitayama T, Taniura H, Yoneda Y (August 2003). "Immersion fixation with Carnoy solution for conventional immunohistochemical detection of particular N-methyl-D-aspartate receptor subunits in murine hippocampus". J. Neurosci. Res. 73 (3): 416–26. doi:10.1002/jnr.10622. PMID 12868075. S2CID 40884078.

[pmid18353202-9] Madras J, Lapointe H (March 2008). "Keratocystic odontogenic tumour: reclassification of the odontogenic keratocyst from cyst to tumour". J Can Dent Assoc. 74 (2): 165–165h. PMID 18353202.

[10] "Odontogenic Keratocyst: The Northwestern USA Experience". Archived from the original on 2009-01-06. Retrieved 14 Jan 2009.

[11] "Use of Carnoy's Solution in management of odontogenic keratocysts". Archived from the original on 2009-05-17. Retrieved 14 Jan 2009.

[pmid15290793-12] Lee PK, Samman N, Ng IO (April 2004). "Unicystic ameloblastoma--use of Carnoy's solution after enucleation". Int J Oral Maxillofac Surg. 33 (3): 263–7. doi:10.1006/ijom.2003.0496. PMID 15290793.

[pmid16782308-13] Lau SL, Samman N (August 2006). "Recurrence related to treatment modalities of unicystic ameloblastoma: a systematic review". Int J Oral Maxillofac Surg. 35 (8): 681–90. doi:10.1016/j.ijom.2006.02.016. PMID 16782308.

[isbn0-86715-390-3-14] Marx, Robert E.; Stern, Diane (2003). Oral and maxillofacial pathology: a rationale for diagnosis and treatment. Chicago: Quintessence. p. 684. ISBN 0-86715-390-3.

[pmid18499991-15] Shamsi M, Abdali K, Montazer NR, Kumar PV, Tabatabaee HR (2008). "Comparison of Carnoy's solution and 96% ethyl alcohol fixation in bloody Pap smears". Acta Cytol. 52 (2): 187–90. doi:10.1159/000325477. PMID 18499991. S2CID 3370408.

[pmid16260588-16] Miething F, Hering S, Hanschke B, Dressler J (March 2006). "Effect of fixation to the degradation of nuclear and mitochondrial DNA in different tissues". J. Histochem. Cytochem. 54 (3): 371–4. doi:10.1369/jhc.5B6726.2005. PMID 16260588.

[17] "Stains File: Carnoy's Fluid". Archived from the original on 15 December 2010. Retrieved 25 Oct 2009.