Formamidase
| Formamidase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 Formamidase hexamer, Helicobacter pylori | |||||||||
| Identifiers | |||||||||
| EC no. | 3.5.1.49 | ||||||||
| CAS no. | 9013-59-6 | ||||||||
| Databases | |||||||||
| BRENDA | enzyme data | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | enzyme entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| Rhea | reactions | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
Formamidase (EC 3.5.1.49) is an enzyme characterised from Pseudomonas aeruginosa and Alcaligenes eutrophus that catalyzes the chemical reaction which hydrolyses formamide to formic acid and ammonia:
The formamide binding site in Helicobacter pylori consists of a cysteine-glutamate-lysine catalytic triad. Metabolic engineering techniques have been used to create organisms that can use formamide as sole nitrogen source and hence manufacture useful organic amines from it.
This enzyme is a hydrolase, one acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is formamide amidohydrolase.
Structural studies
As of late 2007, four structures have been solved for this class of enzymes, with PDB accession codes PDB: 2DYU, PDB: 2DYV, PDB: 2E2K, and PDB: 2E2L.
References
- ↑ Clarke, Patricia H. (1969). "The Aliphatic Amidases of Pseudomonas aeruginosa". Advances in Microbial Physiology Volume 4. Vol. 4. pp. 179–222. doi:10.1016/S0065-2911(08)60442-7. ISBN 978-0-12-027704-9.
- ↑ Friedrich, C. G.; Mitrenga, G. (1981). "Utilization of Aliphatic Amides and Formation of Two Different Amidases by Alcaligenes eutrophus". Microbiology. 125 (2): 367–374. doi:10.1099/00221287-125-2-367.
- ↑ Hung, Chiu-Lien; Liu, Jia-Hsin; Chiu, Wei-Chun; Huang, Shao-Wei; Hwang, Jenn-Kang; Wang, Wen-Ching (2007). "Crystal Structure of Helicobacter pylori Formamidase AmiF Reveals a Cysteine-Glutamate-Lysine Catalytic Triad". Journal of Biological Chemistry. 282 (16): 12220–12229. doi:10.1074/jbc.M609134200. PMID 17307742.
- ↑ Schwardmann, Lynn S.; Wu, Tong; Dransfeld, Aron K.; Lindner, Steffen N.; Wendisch, Volker F. (2023). "Formamide-based production of amines by metabolically engineering Corynebacterium glutamicum". Applied Microbiology and Biotechnology. 107 (13): 4245–4260. doi:10.1007/s00253-023-12592-3. PMC 10313556. PMID 37246985.
- ↑ Enzyme 3.5.1.49 at KEGG Pathway Database.
