GTP cyclohydrolase IIa

GTP cyclohydrolase IIa
Identifiers
EC no.3.5.4.29
Databases
BRENDAenzyme data
ExPASyNiceZyme view
KEGGenzyme entry
MetaCycmetabolic pathway
Rheareactions
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
Search
PMCarticles
PubMedarticles
NCBIproteins

GTP cyclohydrolase IIa (EC 3.5.4.29) is an enzyme that catalyzes the hydrolysis of guanosine triphosphate to 2-amino-5-formylamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one (1) and orthophosphate Pi:

2D representation of the chemical structure of guanosine triphosphate.
guanosine triphosphate
 
3 H2O
2 Pi
Rightward reaction arrow with minor substrate(s) from top left and minor product(s) to top right
 
 
 
2D representation of the chemical structure of 2-Amino-5-formylamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one.
(1)

The reaction is part of the biosynthetic pathway to riboflavin in bacteria.

This enzyme is a hydrolase, one acting on carbon-nitrogen bonds other than peptide bonds, specifically in cyclic amidines. The systematic name of this enzyme class is GTP 8,9-hydrolase (phosphate-forming).

References

  1. ↑ Graham DE, Xu H, White RH (2002). "A member of a new class of GTP cyclohydrolases produces formylaminopyrimidine nucleotide monophosphates". Biochemistry. 41 (50): 15074–84. doi:10.1021/bi0268798. PMID 12475257.
  2. ↑ Schomburg, Dietmar; Schomburg, Ida (2013). "2-amino-5-formylamino-6-ribosylaminopyrimidin-4(3H)-one 5'-monophosphate deformylase 3.5.1.102". Class 3.4–6 Hydrolases, Lyases, Isomerases, Ligases. pp. 244–246. doi:10.1007/978-3-642-36260-6_13. ISBN 978-3-642-36259-0.
  3. ↑ Liu, Shuang; Hu, Wenya; Wang, Zhiwen; Chen, Tao (2020). "Production of riboflavin and related cofactors by biotechnological processes". Microbial Cell Factories. 19 (1) 31. doi:10.1186/s12934-020-01302-7. PMC 7017516. PMID 32054466.
  4. ↑ Enzyme 3.5.4.29 at KEGG Pathway Database.