SR135 begins at the Florida state line intersecting with County Road 141 (CR141) south. In Echols County it intersects SR94, and SR376. SR135 enters Lowndes County east of Valdosta. The only major junction in the county is with US84/SR38. SR135 then enters Lanier County south of Stoctkon. In downtown, it travels concurrently with US221/SR31 for a short distance. North of Lakeland, it travels concurrently with SR64. SR135 enters southeast of Nashville. Just north of the county line, SR64 splits off and joins SR168. SR135 also intersects SR76, which terminates at SR135. SR135 enters Atkinson County south of Willacoochee. In Willacoochee, it travels concurrently for a short distance with US82 and SR520. SR135 enters Coffee County south of Douglas, where it also travels parallel to a former Central of Georgia Railway right-of-way. In Douglas, it travels concurrently with SR206 before the latter terminates. At the terminus, SR135 travels concurrently with US221 again. Together, they intersect SR32, SR158, and SR206 Conn. before leaving Douglas. SR135 enters Jeff Davis County southwest of Hazlehurst. It intersects SR107 just north of Denton; SR107 terminates at SR135. In Hazlehurst, US221 splits off for a short way, while SR135 picks up SR135 Conn. SR135 then picks US221 back up, heading north. They then travel concurrently with US23, US341, SR19, and SR27. After intersecting SR19 Conn, these roads split off. SR135 enters Montgomery County south of Uvalda. In Uvalda, US221 splits off at an intersection with SR56. North of Uvalda, SR135 intersects SR130. In Higgston, SR135 terminates at US280, SR15, SR29, and SR30.
State Route135 Loop (SR135 Loop) is a 2.5-mile-long (4.0km)unsigned loop route south of the city limits of Lakeland, consisting entirely of Burnt Church Road. It begins at an intersection with SR135 south of Darsey Pond, then curves to the northeast. At Union Church and the cemetery across the street from it, the road begins to curve to the northwest and then travels to the west just south of the Lakeland city limits. The highway intersects SR135 Byp. (South Oak Street) before finally terminating at another intersection with the SR135 mainline (South Mill Street). Burnt Church Road continues to the west as an unnumbered street leading to US221.
State Route135 Bypass (SR135 Byp.) is a 1.60-mile-long (2.57km)bypass route of SR135 that is mostly within the city limits of Lakeland. It begins at an intersection with the SR135 mainline just south of the Lakeland city limits, then intersects SR135 Loop (only signed as Burnt Church Road) before entering the city. SR135 Byp. intersects US129/US221/SR11/SR31/SR37/SR122 (Main Street) and travels concurrently with them for two blocks, then turns north onto North College Street in a one-block concurrency with SR11 Byp., which turns left at East Church Avenue. SR135 Byp. ends at another intersection with the SR135 mainline near the Charles Knight Cemetery.
State Route135 Connector (SR135 Conn.) is a 0.73-mile-long (1.17km)connecting route of SR135 that exists mostly within the city limits of Hazlehurst. It connects US23/SR19 (Alma Highway/Larry Contos Boulevard) with US221/SR135 (Jefferson Street/Cromartie Street). It consists of a southern part of Jefferson Street. The highway is entirely concurrent with US221 Truck / SR135 Truck.
State Route135 Truck (SR135 Truck) is a 2.0-mile-long (3.2km)truck route of SR135 that exists mostly within the city limits of Hazlehurst. It is entirely concurrent with US221 Truck. It begins at an intersection with US221/SR135 (Jefferson Street/Cromartie Street). It travels to the southeast on Jefferson Street, concurrent with US221 Truck and SR135 Conn., which both begin at this intersection, as well. The three highways cross over some railroad tracks of Norfolk Southern Railway (NS) and then leave the city limits. They intersect US23/SR19 (Alma Highway). Here, SR135 meets its southern terminus, and US221 Truck and SR135 Truck turn left onto US23/SR19 onto Larry Contos Boulevard. The four highways travel to the north-northeast. Just before passing a Walmart Supercenter, they re-enter Hazlehurst. They cross over some railroad tracks of NS Railway and then intersect US341/SR27 (Golden Isles Parkway). Here, all six highways travel to the northwest. At Plum Street, the concurrency intersects the northbound lanes of US221/SR135 (since those highways travel through the city on one-way streets). At this intersection, US221 Truck and SR135 Truck meet their northern terminus.
CR 141 at the Florida state line near Jennings, Florida
US 280 / SR 15 / SR 29 / SR 30 in Higgston
