Theta Chi fraternity has chartered 245 chapters, of which 150 are active as of 2024. Its first 24 chapters were given single Greek letter designations (Alpha, Beta, etc.), and all following chapters were given double Greek letter designations (Alpha Beta, Alpha Gamma, etc.) with no use of double letters (no Alpha Alpha, Beta Beta, etc.).
Following is a list of Theta Chi chapters, with active chapters indicated in bold and inactive chapters and institutions in italics.