DNA polymerase iota
DNA polymerase iota is an enzyme that in humans is encoded by the POLI gene. It is found in higher eukaryotes, and is believed to have arisen from a gene duplication from Pol η. Pol ι, is a Y family polymerase that is involved in translesion synthesis. It can bypass 6-4 pyrimidine adducts and abasic sites and has a high frequency of wrong base incorporation. Like many other Y family polymerases, Pol ι has low processivity, a large DNA binding pocket, and doesn't undergo conformational changes when DNA binds. These attributes are what allow Pol ι to carry out its task as a translesion polymerase. Pol ι only uses Hoogsteen base pairing, flipping the purines adenine and guanine to the syn conformations. During DNA synthesis, it will add thymine opposite to adenine and can add both cytosine and thymine opposite to guanine.
Xeroderma pigmentosum variant
Xeroderma pigmentosum variant (XPV) cells lack DNA polymerase eta (η). Instead these cells use DNA polymerase iota (ι). Exposure of XPV cells to UV light causes a very high frequency and unique spectrum of UV-induced mutations that can ultimately lead to malignant transformation.
References
- 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000038425 – Ensembl, May 2017
- ↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ↑ Frank EG, Woodgate R (August 2007). "Increased catalytic activity and altered fidelity of human DNA polymerase iota in the presence of manganese". The Journal of Biological Chemistry. 282 (34): 24689–24696. doi:10.1074/jbc.M702159200. PMID 17609217.
- ↑ Nair DT, Johnson RE, Prakash L, Prakash S, Aggarwal AK (July 2006). "Hoogsteen base pair formation promotes synthesis opposite the 1,N6-ethenodeoxyadenosine lesion by human DNA polymerase iota". Nature Structural & Molecular Biology. 13 (7): 619–625. doi:10.1038/nsmb1118. PMID 16819516.
- 1 2 3 Wang Y, Woodgate R, McManus TP, Mead S, McCormick JJ, Maher VM (1 April 2007). "Evidence that in xeroderma pigmentosum variant cells, which lack DNA polymerase eta, DNA polymerase iota causes the very high frequency and unique spectrum of UV-induced mutations". Cancer Research. 67 (7): 3018–3026. doi:10.1158/0008-5472.can-06-3073. PMID 17409408.
Further reading
- McIntyre J (2020). "Polymerase iota - an odd sibling among Y family polymerases". DNA Repair. 86 102753. Amst. doi:10.1016/j.dnarep.2019.102753. PMID 31805501.
- McDonald JP, Tissier A, Frank EG, Iwai S, Hanaoka F, Woodgate R (January 2001). "DNA polymerase iota and related rad30-like enzymes". Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences. 356 (1405): 53–60. doi:10.1098/rstb.2000.0748. PMC 1087691. PMID 11205331.
- Tissier A, Frank EG, McDonald JP, Vaisman A, Fernàndez de Henestrosa AR, Boudsocq F, et al. (May 2001). "Biochemical characterization of human DNA polymerase iota provides clues to its biological function". Biochemical Society Transactions. 29 (Pt 2): 183–187. doi:10.1042/BST0290183. PMID 11356150.
- McDonald JP, Rapić-Otrin V, Epstein JA, Broughton BC, Wang X, Lehmann AR, et al. (August 1999). "Novel human and mouse homologs of Saccharomyces cerevisiae DNA polymerase eta". Genomics. 60 (1): 20–30. doi:10.1006/geno.1999.5906. PMID 10458907.
- Masutani C, Kusumoto R, Iwai S, Hanaoka F (2000). "Mechanisms of accurate translesion synthesis by human DNA polymerase eta". The EMBO Journal. 19 (12): 3100–3109. doi:10.1093/emboj/19.12.3100. PMC 203367. PMID 10856253.
- Tissier A, McDonald JP, Frank EG, Woodgate R (2000). "poliota, a remarkably error-prone human DNA polymerase". Genes & Development. 14 (13): 1642–1650. doi:10.1101/gad.14.13.1642. PMC 316739. PMID 10887158.
- Tissier A, Frank EG, McDonald JP, Iwai S, Hanaoka F, Woodgate R (October 2000). "Misinsertion and bypass of thymine-thymine dimers by human DNA polymerase iota". The EMBO Journal. 19 (19): 5259–5266. doi:10.1093/emboj/19.19.5259. PMC 302107. PMID 11013228.
- Hartley JL, Temple GF, Brasch MA (2001). "DNA cloning using in vitro site-specific recombination". Genome Research. 10 (11): 1788–1795. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
- Wiemann S, Weil B, Wellenreuther R, Gassenhuber J, Glassl S, Ansorge W, et al. (March 2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome Research. 11 (3): 422–435. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.
- Bebenek K, Tissier A, Frank EG, McDonald JP, Prasad R, Wilson SH, et al. (March 2001). "5'-Deoxyribose phosphate lyase activity of human DNA polymerase iota in vitro". Science. 291 (5511). New York, N.Y.: 2156–2159. Bibcode:2001Sci...291.2156B. doi:10.1126/science.1058386. PMID 11251121. S2CID 83716303.
- Simpson JC, Wellenreuther R, Poustka A, Pepperkok R, Wiemann S (September 2000). "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing". EMBO Reports. 1 (3): 287–292. doi:10.1093/embo-reports/kvd058. PMC 1083732. PMID 11256614.
- Frank EG, Tissier A, McDonald JP, Rapić-Otrin V, Zeng X, Gearhart PJ, et al. (June 2001). "Altered nucleotide misinsertion fidelity associated with poliota-dependent replication at the end of a DNA template". The EMBO Journal. 20 (11): 2914–2922. doi:10.1093/emboj/20.11.2914. PMC 125476. PMID 11387224.
- Vaisman A, Tissier A, Frank EG, Goodman MF, Woodgate R (August 2001). "Human DNA polymerase iota promiscuous mismatch extension". The Journal of Biological Chemistry. 276 (33): 30615–30622. doi:10.1074/jbc.M102694200. PMID 11402031.
- Ohmori H, Friedberg EC, Fuchs RP, Goodman MF, Hanaoka F, Hinkle D, et al. (July 2001). "The Y-family of DNA polymerases". Molecular Cell. 8 (1): 7–8. doi:10.1016/S1097-2765(01)00278-7. PMID 11515498.
- Vaisman A, Woodgate R (2002). "Unique misinsertion specificity of poliota may decrease the mutagenic potential of deaminated cytosines". The EMBO Journal. 20 (22): 6520–6529. doi:10.1093/emboj/20.22.6520. PMC 125734. PMID 11707422.
- Faili A, Aoufouchi S, Flatter E, Guéranger Q, Reynaud CA, Weill JC (October 2002). "Induction of somatic hypermutation in immunoglobulin genes is dependent on DNA polymerase iota". Nature. 419 (6910): 944–947. Bibcode:2002Natur.419..944F. doi:10.1038/nature01117. PMID 12410315. S2CID 4431410.
- Kannouche P, Fernández de Henestrosa AR, Coull B, Vidal AE, Gray C, Zicha D, et al. (November 2002). "Localization of DNA polymerases eta and iota to the replication machinery is tightly co-ordinated in human cells". The EMBO Journal. 21 (22): 6246–6256. doi:10.1093/emboj/cdf618. PMC 137208. PMID 12426396.
- Frank EG, Sayer JM, Kroth H, Ohashi E, Ohmori H, Jerina DM, et al. (December 2002). "Translesion replication of benzo[a]pyrene and benzo[c]phenanthrene diol epoxide adducts of deoxyadenosine and deoxyguanosine by human DNA polymerase iota". Nucleic Acids Research. 30 (23): 5284–5292. doi:10.1093/nar/gkf643. PMC 137958. PMID 12466554.
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