SF3A1 Protein-coding gene in the species Homo sapiens
Splicing factor 3 subunit 1 is a protein that in humans is encoded by the SF3A1 gene .
This gene encodes subunit 1 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 1 belongs to the SURP protein family; named for the SURP (also called SWAP or Suppressor-of-White-APricot) motifs that are thought to mediate RNA binding. Subunit 1 has tandemly repeated SURP motifs in its amino-terminal half while its carboxy-terminal half contains a proline-rich region and a ubiquitin-like domain. Binding studies with truncated subunit 1 derivatives demonstrated that the two SURP motifs are necessary for binding to subunit 3 while contacts with subunit 2 may occur through sequences carboxy-terminal to the SURP motifs. Alternative splicing results in multiple transcript variants encoding different isoforms.
References
1 2 3 GRCh38: Ensembl release 89: ENSG00000099995 – Ensembl , May 20171 2 3 GRCm38: Ensembl release 89: ENSMUSG00000002129 – Ensembl , May 2017↑ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .↑ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .↑ Krämer A, Mulhauser F, Wersig C, Gröning K, Bilbe G (May 1995). "Mammalian splicing factor SF3a120 represents a new member of the SURP family of proteins and is homologous to the essential splicing factor PRP21p of Saccharomyces cerevisiae" . RNA . 1 (3). New York, N.Y.: 260– 272. PMC 1369079 PMID 7489498 . 1 2 "Entrez Gene: SF3A1 splicing factor 3a, subunit 1, 120kDa" .↑ Nesic D, Krämer A (October 2001). "Domains in human splicing factors SF3a60 and SF3a66 required for binding to SF3a120, assembly of the 17S U2 snRNP, and prespliceosome formation" . Molecular and Cellular Biology . 21 (19): 6406– 6417. doi :10.1128/MCB.21.19.6406-6417.2001 . PMC 99788 PMID 11533230 . ↑ Chiara MD, Champion-Arnaud P, Buvoli M, Nadal-Ginard B, Reed R (July 1994). "Specific protein-protein interactions between the essential mammalian spliceosome-associated proteins SAP 61 and SAP 114" . Proceedings of the National Academy of Sciences of the United States of America . 91 (14): 6403– 6407. Bibcode :1994PNAS...91.6403C . doi :10.1073/pnas.91.14.6403 PMC 44210 PMID 8022796 . ↑ Ajuh P, Kuster B, Panov K, Zomerdijk JC, Mann M, Lamond AI (December 2000). "Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry" . The EMBO Journal . 19 (23): 6569– 6581. doi :10.1093/emboj/19.23.6569 . PMC 305846 PMID 11101529 .
Further reading
Rain JC, Tartakoff AM, Krämer A, Legrain P (June 1996). "Essential domains of the PRP21 splicing factor are implicated in the binding to PRP9 and PRP11 proteins and are conserved through evolution" . RNA . 2 (6). New York, N.Y.: 535– 550. PMC 1369393 PMID 8718683 . Neubauer G, King A, Rappsilber J, Calvio C, Watson M, Ajuh P, et al. (September 1998). "Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex". Nature Genetics . 20 (1): 46– 50. doi :10.1038/1700 . PMID 9731529 . S2CID 585778 . Sanger Centre T, Washington University Genome Sequencing Cente T (November 1998). "Toward a complete human genome sequence" . Genome Research . 8 (11): 1097– 1108. doi :10.1101/gr.8.11.1097 PMID 9847074 . Krämer A, Grüter P, Gröning K, Kastner B (June 1999). "Combined biochemical and electron microscopic analyses reveal the architecture of the mammalian U2 snRNP" . The Journal of Cell Biology . 145 (7): 1355– 1368. doi :10.1083/jcb.145.7.1355 . PMC 2133165 PMID 10385517 . Das R, Zhou Z, Reed R (May 2000). "Functional association of U2 snRNP with the ATP-independent spliceosomal complex E" . Molecular Cell . 5 (5): 779– 787. doi :10.1016/S1097-2765(00)80318-4 PMID 10882114 . Gunther M, Laithier M, Brison O (July 2000). "A set of proteins interacting with transcription factor Sp1 identified in a two-hybrid screening". Molecular and Cellular Biochemistry . 210 (1– 2): 131– 142. doi :10.1023/A:1007177623283 . PMID 10976766 . S2CID 1339642 . Suzuki Y, Tsunoda T, Sese J, Taira H, Mizushima-Sugano J, Hata H, et al. (May 2001). "Identification and characterization of the potential promoter regions of 1031 kinds of human genes" . Genome Research . 11 (5): 677– 684. doi :10.1101/gr.gr-1640r . PMC 311086 PMID 11337467 . Will CL, Schneider C, MacMillan AM, Katopodis NF, Neubauer G, Wilm M, et al. (August 2001). "A novel U2 and U11/U12 snRNP protein that associates with the pre-mRNA branch site" . The EMBO Journal . 20 (16): 4536– 4546. doi :10.1093/emboj/20.16.4536 . PMC 125580 PMID 11500380 . Jurica MS, Licklider LJ, Gygi SR, Grigorieff N, Moore MJ (April 2002). "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis" . RNA . 8 (4). New York, N.Y.: 426– 439. doi :10.1017/S1355838202021088 . PMC 1370266 PMID 11991638 . Will CL, Urlaub H, Achsel T, Gentzel M, Wilm M, Lührmann R (September 2002). "Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein" . The EMBO Journal . 21 (18): 4978– 4988. doi :10.1093/emboj/cdf480 . PMC 126279 PMID 12234937 . Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, et al. (August 2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins" . Proceedings of the National Academy of Sciences of the United States of America . 101 (33): 12130– 12135. Bibcode :2004PNAS..10112130B . doi :10.1073/pnas.0404720101 PMC 514446 PMID 15302935 . Nesic D, Tanackovic G, Krämer A (September 2004). "A role for Cajal bodies in the final steps of U2 snRNP biogenesis" . Journal of Cell Science . 117 (Pt 19): 4423– 4433. doi :10.1242/jcs.01308 PMID 15316075 . Lin KT, Lu RM, Tarn WY (October 2004). "The WW domain-containing proteins interact with the early spliceosome and participate in pre-mRNA splicing in vivo" . Molecular and Cellular Biology . 24 (20): 9176– 9185. doi :10.1128/MCB.24.20.9176-9185.2004 . PMC 517884 PMID 15456888 . Collins JE, Wright CL, Edwards CA, Davis MP, Grinham JA, Cole CG, et al. (2004). "A genome annotation-driven approach to cloning the human ORFeome" . Genome Biology . 5 (10) R84. doi :10.1186/gb-2004-5-10-r84 PMC 545604 PMID 15461802 .
External links
PDB gallery
1we7 : Solution structure of Ubiquitin-like domain in SF3a120
1zkh : Solution structure of a human ubiquitin-like domain in SF3A1
2dt6 : Solution structure of the first SURP domain of human splicing factor SF3a120
2dt7 : Solution structure of the second SURP domain of human splicing factor SF3a120 in complex with a fragment of human splicing factor SF3a60
