STRAD alpha

STRADA
Identifiers
Aliases	STRADA, LYK5, NY-BR-96, PMSE, STRAD, Stlk, STE20-related kinase adaptor alpha, STRAD alpha, STE20 related adaptor alpha
External IDs	OMIM: 608626; MGI: 1919399; GeneCards: STRADA
Available structures
PDB	Ortholog search: PDBe RCSB
List of PDB id codes
	1UPK, 2WTK, 3GNI
Gene location (Human)
Chr.	Chromosome 17 (human)
End	63,741,986 bp
Gene location (Mouse)
Chr.	Chromosome 11 (mouse)
End	106,092,994 bp
RNA expression pattern
	Top expressed in
	right uterine tube; ; gastric mucosa; ; left testis; ; right testis; ; tibial nerve; ; right hemisphere of cerebellum; ; right frontal lobe; ; ganglionic eminence; ; right ovary; ; body of uterus;
	Top expressed in
	spermatocyte; ; seminiferous tubule; ; spermatid; ; neural layer of retina; ; morula; ; granulocyte; ; tail of embryo; ; zygote; ; ventricular zone; ; superior frontal gyrus;
	More reference expression data
	; ;
	More reference expression data
Gene ontology
Molecular function	protein binding; protein serine/threonine kinase activator activity; protein kinase activator activity; protein kinase activity; ATP binding; nucleotide binding; kinase binding; protein serine/threonine kinase activity;
Cellular component	nucleoplasm; nucleus; cytoplasm; cytosol;
Biological process	protein export from nucleus; cell cycle; protein phosphorylation; positive regulation of protein serine/threonine kinase activity; activation of protein kinase activity; regulation of mitotic cell cycle; stress-activated protein kinase signaling cascade; regulation of apoptotic process; MAPK cascade; signal transduction; neuron projection morphogenesis;
	Sources:Amigo / QuickGO
Orthologs
Databases	NCBI: entry; OMA: entry
	92335
	72149
	ENSG00000266173
	ENSMUSG00000069631
	Q7RTN6
	Q3UUJ4
NM_001003786; NM_001003787; NM_001003788; NM_001165969; NM_001165970;
	NM_153335; NM_001363786; NM_001363787; NM_001363788; NM_001363789; NM_001363790; NM_001363791
	NM_001252448; NM_001252449; NM_028126
NP_001003786; NP_001003787; NP_001003788; NP_001159441; NP_001159442;
	NP_699166; NP_001350715; NP_001350716; NP_001350717; NP_001350718; NP_001350719; NP_001350720
	NP_001239377; NP_001239378; NP_082402
	Wikidata
View/Edit Human	View/Edit Mouse

STRAD alpha (or STRADα) is a protein that in humans is encoded by the gene STRADA (previously LYK5).

Function

Endogenous LKB1 and STRADα form a complex in which STRADα activates LKB1, resulting in phosphorylation of both partners. Removal of endogenous LYK5 by small interfering RNA abrogates LKB1-induced G1 phase arrest. STRADα stabilizes LKB1 protein both in vivo and in vitro, and is capable of eliciting multiple axons in mouse embryonic cortical cultured neurons when overexpressed with LKB1. STRADα is highly spliced in vivo, and this is both developmentally regulated and tissue-specific, but the unique functions of the splice variants are not yet understood.

Disease linkage

Mutations in the LYK5/STRADα gene are associated with polyhydramnios, megalencephaly and symptomatic epilepsy (collectively known as the PMSE syndrome).

Interactions

STRADα has been shown to interact with LKB1 and MO25.

References

1 2 3 GRCh38: Ensembl release 89: ENSG00000266173 – Ensembl, May 2017
1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000069631 – Ensembl, May 2017
↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ "Entrez Gene: LYK5 protein kinase LYK5".
1 2 3 Baas AF, Boudeau J, Sapkota GP, Smit L, Medema R, Morrice NA, et al. (June 2003). "Activation of the tumour suppressor kinase LKB1 by the STE20-like pseudokinase STRAD". The EMBO Journal. 22 (12): 3062–3072. doi:10.1093/emboj/cdg292. PMC 162144. PMID 12805220.
↑ Marignani PA, Scott KD, Bagnulo R, Cannone D, Ferrari E, Stella A, et al. (October 2007). "Novel splice isoforms of STRADalpha differentially affect LKB1 activity, complex assembly and subcellular localization". Cancer Biology & Therapy. 6 (10): 1627–1631. doi:10.4161/cbt.6.10.4787. PMID 17921699.
↑ Veleva-Rotse BO, Smart JL, Baas AF, Edmonds B, Zhao ZM, Brown A, et al. (2014). "STRAD pseudokinases regulate axogenesis and LKB1 stability". Neural Development. 9 (5) 5. doi:10.1186/1749-8104-9-5. PMC 4016016. PMID 24594058./
↑ Puffenberger EG, Strauss KA, Ramsey KE, Craig DW, Stephan DA, Robinson DL, et al. (July 2007). "Polyhydramnios, megalencephaly and symptomatic epilepsy caused by a homozygous 7-kilobase deletion in LYK5". Brain. 130 (Pt 7): 1929–1941. doi:10.1093/brain/awm100. PMID 17522105.
↑ Boudeau J, Scott JW, Resta N, Deak M, Kieloch A, Komander D, et al. (December 2004). "Analysis of the LKB1-STRAD-MO25 complex". Journal of Cell Science. 117 (Pt 26): 6365–6375. doi:10.1242/jcs.01571. PMID 15561763.

Baas AF, Smit L, Clevers H (2004). "LKB1 tumor suppressor protein: PARtaker in cell polarity". Trends in Cell Biology. 14 (6): 312–319. doi:10.1016/j.tcb.2004.04.001. PMID 15183188.
Scanlan MJ, Gout I, Gordon CM, Williamson B, Stockert E, Gure AO, et al. (2003). "Humoral immunity to human breast cancer: antigen definition and quantitative analysis of mRNA expression". Cancer Immunity. 1: 4. PMID 12747765.
Boudeau J, Baas AF, Deak M, Morrice NA, Kieloch A, Schutkowski M, et al. (2003). "MO25alpha/beta interact with STRADalpha/beta enhancing their ability to bind, activate and localize LKB1 in the cytoplasm". The EMBO Journal. 22 (19): 5102–5114. doi:10.1093/emboj/cdg490. PMC 204473. PMID 14517248.
Milburn CC, Boudeau J, Deak M, Alessi DR, van Aalten DM (2004). "Crystal structure of MO25 alpha in complex with the C terminus of the pseudo kinase STE20-related adaptor". Nature Structural & Molecular Biology. 11 (2): 193–200. doi:10.1038/nsmb716. PMID 14730349. S2CID 18401688.
Lizcano JM, Göransson O, Toth R, Deak M, Morrice NA, Boudeau J, et al. (2005). "LKB1 is a master kinase that activates 13 kinases of the AMPK subfamily, including MARK/PAR-1". The EMBO Journal. 23 (4): 833–843. doi:10.1038/sj.emboj.7600110. PMC 381014. PMID 14976552.

This article on a gene on human chromosome 17 is a stub. You can help Wikipedia by adding missing information.

[refGRCh38Ensembl-1] 1 2 3 GRCh38: Ensembl release 89: ENSG00000266173 – Ensembl, May 2017

[refGRCm38Ensembl-2] 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000069631 – Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[entrez-5] "Entrez Gene: LYK5 protein kinase LYK5".

[Baas_2003-6] 1 2 3 Baas AF, Boudeau J, Sapkota GP, Smit L, Medema R, Morrice NA, et al. (June 2003). "Activation of the tumour suppressor kinase LKB1 by the STE20-like pseudokinase STRAD". The EMBO Journal. 22 (12): 3062–3072. doi:10.1093/emboj/cdg292. PMC 162144. PMID 12805220.

[7] Marignani PA, Scott KD, Bagnulo R, Cannone D, Ferrari E, Stella A, et al. (October 2007). "Novel splice isoforms of STRADalpha differentially affect LKB1 activity, complex assembly and subcellular localization". Cancer Biology & Therapy. 6 (10): 1627–1631. doi:10.4161/cbt.6.10.4787. PMID 17921699.

[8] Veleva-Rotse BO, Smart JL, Baas AF, Edmonds B, Zhao ZM, Brown A, et al. (2014). "STRAD pseudokinases regulate axogenesis and LKB1 stability". Neural Development. 9 (5) 5. doi:10.1186/1749-8104-9-5. PMC 4016016. PMID 24594058./

[Puffenberger_2007-9] Puffenberger EG, Strauss KA, Ramsey KE, Craig DW, Stephan DA, Robinson DL, et al. (July 2007). "Polyhydramnios, megalencephaly and symptomatic epilepsy caused by a homozygous 7-kilobase deletion in LYK5". Brain. 130 (Pt 7): 1929–1941. doi:10.1093/brain/awm100. PMID 17522105.

[Boudeau_2004-10] Boudeau J, Scott JW, Resta N, Deak M, Kieloch A, Komander D, et al. (December 2004). "Analysis of the LKB1-STRAD-MO25 complex". Journal of Cell Science. 117 (Pt 26): 6365–6375. doi:10.1242/jcs.01571. PMID 15561763.

STRAD alpha

Function

Disease linkage

Interactions

References

Further reading