Christoph Sommer, Christoph Straehle, Thorben Kröger, Bernhard X. Kausler, Ullrich Koethe, Fred A. Hamprecht, Anna Kreshuk, Dominik Kutra, Stuart Berg, Benedikt Best, Maksim Novikov, Emil Melnikov, Tomaz Vieira and others
ilastik allows user to annotate an arbitrary number of classes in images with a mouse interface. Using these user annotations and the generic (nonlinear) image features, the user can train a random forest classifier.
Trained ilastik classifiers can be applied new data not included in the training set in ilastik via its batch processing functionality, or without using the graphical user interface, in headless mode. ilastik can be integrated into various related tools:
Pre-trained workflows can be executed directly from ImageJ/Fiji using the ilastik-ImageJ plugin.
Pre-trained ilastik Pixel Classification workflows can be run directly in Python with the ilastik Python package, which is available via conda.
ilastik has a CellProfiler module to use ilastik classifiers to process images within a CellProfiler framework.
History
ilastik was first released in 2011 by scientists at the Heidelberg Collaboratory for Image Processing (HCI), University of Heidelberg.
Application
The Interactive Learning and Segmentation Toolkit
Carving
Cell classification and neuron classification
Synapse detection
Cell tracking
Neural Network Classification
Resources
ilastik project is hosted on GitHub. It is a collaborative project, any contributions such as comments, bug reports, bug fixes or code contributions are welcome. The ilastik team can be contacted for user support on the image.sc forum.
References
↑Sommer, C; Straehle C; Koethe U; Hamprecht FA (2011). "Ilastik: Interactive learning and segmentation toolkit". 2011 IEEE International Symposium on Biomedical Imaging: From Nano to Macro. pp.230–33. doi:10.1109/ISBI.2011.5872394. ISBN978-1-4244-4127-3. S2CID206949135.