Teams and riders
| Entrant | Constructor | Chassis | Race rider | |
|---|---|---|---|---|
| No. | rider name | |||
 Aprilia Racing |
Aprilla | RS-GP24 | 12 |  Maverick Viñales |
| 41 | Aleix Espargaró | |||
 Trackhouse Racing |
RS-GP23 | 25 | Raúl Fernández | |
| RS-GP24 | 88 |  Miguel Oliveira | ||
| Ducati Lenovo Team |
Ducati | Desmosedici GP24 | 1 | Francesco Bagnaia |
| 23 | Enea Bastianini | |||
| Prima Pramac Racing |
21 | Franco Morbidelli | ||
| 89 | Jorge Martín | |||
| Gresini Racing MotoGP |
Desmosedici GP23 | 73 | Alex Márquez | |
| 93 | Marc Márquez | |||
| Pertamina Enduro VR46 Racing Team |
49 | Fabio Di Giannantanio | ||
| 72 | Marco Bezzecchi | |||
 LCR Honda Castrol |
Honda | RC213V | 5 |  Johann Zarco |
| LCR Honda Idemitsu |
30 |  Takaaki Nakagami | ||
| Repsol Honda Team |
10 | Luca Marini | ||
| 36 | Joan Mir | |||
| Red Bull GasGas Tech3 |
KTM | RC16 | 31 | Pedro Acosta |
| 37 | Augusto Fernández | |||
 Red Bull KTM Factory Racing |
33 |  Brad Binder | ||
| 43 |  Jack Miller | |||
| Monster Energy Yamaha MotoGP |
Yamaha | YZR-M1 | 20 | Fabio Quartararo |
| 42 | Álex Rins | |||
| 87 | Remy Gardner | |||
All teams use series-specified Michelin tyres.
Team changes
RNF Racing will not enter the 2024 season after being found breaching the participation agreement. The replacement is yet to be decided. Trackhouse Racing MotoGP was later announced as the replacement for RNF Racing, taking over as Aprilia's independent team. and retaining riders Miguel Oliveira and Raúl Fernández from RNF.
Rider changes
- Álex Rins will move to Monster Energy Yamaha MotoGP from LCR Honda, replacing Franco Morbidelli who will move to Prima Pramac Racing, replacing Johann Zarco.
- Johann Zarco will move to LCR Honda from Prima Pramac Racing, replacing Álex Rins. Zarco previously raced with the team as a replacement rider in 2019.
- Marc Marquez will move to Gresini Racing from Repsol Honda, replacing Fabio Di Giannantonio who will move to VR46 Racing Team, replacing Luca Marini. Márquez leave Repsol Honda after 11 seasons, winning six Riders' Championships during his stint with the team. and will team up again with his brother Álex, who was his teammate in Repsol Honda in 2020.
- Pedro Acosta will enter the MotoGP class with the Red Bull GasGas Tech3 team, replacing Pol Espargaró who will leave the premier class after 10 seasons.
- Luca Marini will move to Repsol Honda from VR46 Racing Team, replacing Marc Márquez.
Mid-season changes
- Álex Rins will miss the German round after an injury sustained at the Dutch TT and will be replaced by Remy Gardner.
