The examples and perspectives in this article deal primarily with the United States and do not represent a worldwide view of the subject. (July 2026) |
Butterfat or milkfat is the fatty portion of milk. Milk and cream are often sold according to the amount of butterfat they contain.

Butterfat is mainly composed of triglycerides. Each triglyceride contains three fatty acids. Butterfat triglycerides contain the following amounts of fatty acids (by mass fraction):
Butterfat contains about 3% trans fat, which is slightly less than 0.5 grams per US tablespoon. Trans fats occur naturally in meat and milk from ruminants. The predominant kind of trans fat found in milk is vaccenic fatty acid. Trans fats may be also found in some industrially produced foods, such as shortenings obtained by hydrogenation of vegetable oils. In light of recognized scientific evidence, nutritional authorities consider all trans fats equally harmful for health and recommend that their consumption be reduced to trace amounts. However, two Canadian studies have shown that vaccenic acid could be beneficial compared to vegetable shortenings containing trans fats, or a mixture of pork lard and soy fat, by lowering total LDL and triglyceride levels. A study by the US Department of Agriculture showed that vaccenic acid raises both HDL and LDL cholesterol, whereas industrial trans fats only raise LDL with no beneficial effect on HDL.
Butterfat contains 3 to 4% tributyrin, a triglyceride (fat) and precursor of the short-chain fatty acid butyric acid, and is the richest-known food source of tributyrin.
| Fatty acid | length | mol% (rounded) | 1 | 2 | 3 |
|---|---|---|---|---|---|
| Butyryl | C4 | 12 | 0 | 0 | 100 |
| Caproyl | C6 | 5 | 0 | 7 | 93 |
| Caprylyl | C8 | 2 | 25 | 12 | 63 |
| Capryl | C10 | 4 | 17 | 27 | 56 |
| Lauryl | C12 | 4 | 42 | 53 | 5 |
| Myristyl | C14 | 11 | 29 | 52 | 19 |
| Palmityl | C16 | 24 | 47 | 45 | 8 |
| Hexadecenoyl | C16:1 | 3 | 36 | 46 | 18 |
| Stearyl | C18 | 7 | 49 | 45 | 6 |
| Oleyl | C18:1 | 24 | 42 | 26 | 32 |
| Linoleyl | C18:2 | 3 | 23 | 47 | 31 |
Most countries have standards for minimum fat levels in dairy products.
In the U.S., there are federal standards for butterfat content of dairy products.
{{cite journal}}: CS1 maint: multiple names: authors list (link)A direct source of butyrate is the diet, where it is present at low levels in many fruits and vegetables, but its richest source is from milk fat (butter) which contains 3–4 % butyrate as glycerol esters, termed tributyrin