Creighton was born in Delavan, Illinois, the daughter of Cyrus Murray Creighton and Bertha Baldwin Creighton. Her father was born in Canada. She graduated from Wellesley College in 1929, where she found botanist Margaret Clay Ferguson an encouraging faculty mentor.
She completed her Ph.D. at Cornell University in 1933. At Cornell, she worked in the field of maize cytogenetics with Barbara McClintock. The pair published an influential paper in 1931, in which they described chromosomal crossover for the first time. This paper, part of her Ph.D. research, provided key evidence that chromosomes carried and exchanged genetic information and hence that genes for physical traits are carried on chromosomes.
"A Correlation of Cytological and Genetical Crossing-Over in Zea mays" (1931, with Barbara McClintock)
"Three Cases of Deficiency in Chromosome 9 of Zea mays" (1934)
"Production and Distribution of Growth Hormone in Shoots of Aesculus and Malus, and Its Probable Role in Stimulating Cambial Activity" (1937, with George S. Avery Jr. and Paul R. Burkholder)
"Nutrient Deficiencies and Growth Hormone Concentration in Helianthus and Nicotiana" (1937, with George S. Avery Jr. and Paul R. Burkholder)
"Extraction Methods in Relation to Hormone Content of Maize Endosperms" (1940, with George S. Avery Jr. and Barbara Shalucha)
"Expression of Hormone Yields in Relation to Different Avena Test Methods" (1941, with George S. Avery Jr. and Barbara Shalucha)
Personal life and legacy
Creighton's companion later in life was Gertrude Dever; they met in the WAVES and lived together for many years. Creighton died in 2004, at the age of 94, in Needham, Massachusetts. Wellesley College has a collection of her personal papers. Creighton's close personal and professional relationship with Barbara McClintock was dramatized in a 2023 play by Carolyn Gage, In McClintock's Corn.
Kass, L. B. and Chomet, P. 2009. Barbara McClintock, Pgs. 17–52, in J. Bennetzen and S. Hake, Editors, Handbook of Maize: Genetics and Genomics. Springer
Kalte, Pamela M. and Nemeh, Katherine H. (2005) "Creighton, Harriet Baldwin (1909–)" American Men & Women of Science: A biographical directory of today's leaders in physical, biological and related sciences (22nd ed.) Thomson Gale, Detroit
McGrayne, Sharon Bertsch (1998) Nobel Prize Women in Science: Their Lives, Struggles, and Momentous Discoveries Carol Publishing Group, New Jersey