5-phytase

5-phytase
Identifiers
EC no.3.1.3.72
CAS no.357208-41-4
Databases
BRENDAenzyme data
ExPASyNiceZyme view
KEGGenzyme entry
MetaCycmetabolic pathway
Rheareactions
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
Search
PMCarticles
PubMedarticles
NCBIproteins

The enzyme 5-phytase (EC 3.1.3.72) catalyzes the reaction

2D representation of the chemical structure of phytic acid .
phytic acid
 
H2O
Rightward reaction arrow with minor substrate(s) from top left and minor product(s) to top right
 
 
 
2D representation of the chemical structure of Q27108885.
1L-myo-inositol 1,2,3,4,6-pentakisphosphate

The enzyme characterised from Lilium longiflorum pollen hydrolyses phytic acid (myo-inositol hexakisphosphate) to 1L-myo-inositol 1,2,3,4,6-pentakisphosphate and orthophosphate (Pi). Mechanistically, this is a histidine acid phosphatase. A protein tyrosine phosphatase-like phytase from Selenomonas ruminantium subsp. lactilytica has also been described. Both are specific for the 5-phosphate position of myo-inositol hexakisphosphate.

This enzyme is a hydrolase, specifically one acting on phosphoric monoester bonds. The systematic name of this enzyme class is myo-inositol-hexakisphosphate 5-phosphohydrolase.

Structural studies

As of late 2007, only the phytase purified from lily pollen had its structure solved, with PDB accession codes PDB: 1U24​, PDB: 1U25​, PDB: 1U26​, PDB: 2B4O​, PDB: 2B4P​, and PDB: 2B4U​.

See also

References

  1. ↑ Barrientos L, Scott JJ, Murthy PP (1994). "Specificity of hydrolysis of phytic acid by alkaline phytase from lily pollen". Plant Physiology. 106 (4): 1489–1495. doi:10.1104/pp.106.4.1489. PMC 159689. PMID 7846160.
  2. ↑ Gocheva, Yana; Engibarov, Stephan; Lazarkevich, Irina; Eneva, Rumyana (2023). "Phytases - Types, Sources, and Factors Affecting Their Activity" (PDF). Acta Microbiologica Bulgaria. 39 (3).
  3. ↑ Puhl A, Greiner R, Selinger LB (2008). "A protein tyrosine phosphatase-like inositol polyphosphatase from Selenomonas ruminantium subsp. lactilytica has specificity for the 5-phosphate of myo-inositol hexakisphosphate". The International Journal of Biochemistry & Cell Biology. 40 (10): 2053–2064. doi:10.1016/j.biocel.2008.02.003. PMID 18358762.
  4. ↑ Nezhad, Nima Ghahremani; Rahman, Raja Noor Zaliha Raja Abd; Normi, Yahaya M.; Oslan, Siti Nurbaya; Shariff, Fairolniza Mohd; Leow, Thean Chor (2023). "Isolation, screening and molecular characterization of phytase-producing microorganisms to discover the novel phytase". Biologia. 78 (9): 2527–2537. doi:10.1007/s11756-023-01391-w.
  5. ↑ Enzyme 3.1.3.72 at KEGG Pathway Database.