| Railroad | Quantity | Road numbers | Notes |
| Electro-Motive Division (plant) | 1 | 105 | Built as model DH-2 |
| Electro-Motive Division (demonstrator) | 1 | 800 | to Illinois Terminal Railroad 725 |
| 1 | 801 | to Georgia Northern Railway 801 |
| 1 | 500 | to Cuyahoga Valley Railway 855 |
| Aliquippa and Southern Railroad | 3 | 800–802 | Built with 567BC engines |
| Atlantic Coast Line Railroad | 10 | 50–59 | |
| Bamberger Railroad | 2 | 601–602 | |
| Boston and Maine Railroad | 8 | 800–807 | |
| Canton Railroad | 4 | 40–43 | 42-43 were built with 567C engines |
| Cedar Rapids and Iowa City Railway ("Crandic") | 3 | 91–93 | Built with 567BC engines |
| Chicago and North Western Railway | 1 | 801 | |
| Chicago, St. Paul, Minneapolis and Omaha Railway ("Omaha Road") | 4 | 126–129 | |
| Chicago, Rock Island and Pacific Railroad | 28 | 811–838 | 836-838 were built with 567BC engines. 838 was acquired by Pfizer Incorporated in 1989 and then donated to the Danbury Railway Museum in 2002. |
| Chicago, West Pullman and Southern Railroad | 5 | 42–46 | |
| Cincinnati Union Terminal | 8 | 30–37 | |
| Colorado and Wyoming Railway | 12 | 201–212 | |
| Columbia Iron and Steel | 1 | 1 | |
| Delaware, Lackawanna and Western Railroad | 11 | 501–511 | |
| De Queen and Eastern Railroad | 1 | D-3 | |
| Detroit, Toledo and Ironton Railroad | 2 | 900–901 | |
| Donora Southern Raileoad | 10 | 802–811 | |
| Great Northern Railway | 3 | 98–99, 101 | 98–99 to Burlington Northern. 101 was rebuilt from an NW1 |
| L.G. Everist Incorporated | 1 | 61 | |
| Lake Terminal Railroad | 4 | 821–824 | |
| Lakeside and Marblehead Railroad | 2 | 13–14 | |
| Lehigh Valley Railroad | 29 | 128–129, 250–276 | 256-273 built with dynamic brakes. |
| Louisiana and North West Railroad | 2 | 40–41 | |
| Ludington and Northern Railroad | 1 | 16 | Currently operational as Kendallville Terminal #16. |
| Merchants Despatch Transportation | 1 | 15 | |
| Monessen Southwestern Railway | 2 | 22, 26 | |
| New Orleans Union Passenger Terminal | 3 | 1–3 | Built with 567BC engines |
| New York Central Railroad | 26 | 9602–9627 | To Penn Central then Conrail 8602–8627, 8618 is preserved on the Strasburg Railroad |
| New York Central Railroad (Chicago River and Indiana Railroad) | 2 | 9600–9601 | To Penn Central then Conrail 8600-8601 |
| New York, Chicago and St. Louis Railroad ("Nickel Plate Road") | 8 | 107–114 | |
| North Louisiana and Gulf Railroad | 2 | 37–38 | Built with 567BC engines |
| Pittsburgh Coke and Chemical Company | 1 | 4 | |
| Poplar Ridge Coal | 1 | 801 | |
| Reserve Mining Company | 2 | 1200–1201 | |
| Roscoe, Snyder and Pacific Railway | 1 | 200 | |
| South Shore Railway | 1 | 12 | to Illinois Central Railroad 802 |
| Southern Pacific Company | 20 | 4604–4623 | All built with dynamic brakes; 4622-4623 built with 567BC engines |
| Southern Pacific (Texas and New Orleans Railroad) | 5 | 12–16 | |
| Steelton and Highspire Railroad | 1 | 26 | |
| Tennessee Coal, Iron and Railroad Company | 6 | 750–754, 770 | |
| Texas and Pacific Railway | 8 | 811–818 | |
| Tremont and Gulf Railway | 2 | 75, 77 | to Illinois Central Railroad 800 and 801 |
| Unity Railways | 1 | 53 | |
| United States Army | 41 | 2000–2040 | All 41 served in the Korean War, including the 2019 and 2022 now at the Heart of Dixie Railroad Museum. 2002 is on display at the National Museum of Transportation in Kirkwood, Missouri. |
| Total | 310 | | |