UBE2M

UBE2M

Identifiers

UBE2M, UBC-RS2, UBC12, hUbc12, ubiquitin conjugating enzyme E2 M

External IDs

OMIM: 603173; MGI: 108278; GeneCards: UBE2M

Available structures

Ortholog search: PDBe RCSB

List of PDB id codes
1TT5, 1Y8X, 2NVU, 3TDU, 3TDZ, 4GAO, 4P5O

Enzyme activity
EC #	BRENDA	ExPASy	KEGG	MetaCyc
2.3.2.34	↗	↗	↗	↗

Gene location (Human)

Chr.	Chromosome 19 (human)

Band	19q13.43	Start	58,555,712 bp
Band	19q13.43	End	58,558,954 bp

Gene location (Mouse)

Chr.	Chromosome 7 (mouse)

Band	7 A1\|7 7.73 cM	Start	12,769,047 bp
Band	7 A1\|7 7.73 cM	End	12,772,202 bp

RNA expression pattern

Human

Mouse (ortholog)

Top expressed in

right frontal lobe

anterior cingulate cortex

prefrontal cortex

left testis

apex of heart

anterior pituitary

right testis

muscle of thigh

amygdala

right hemisphere of cerebellum

Top expressed in

superior frontal gyrus

muscle of thigh

spermatocyte

lip

spermatid

ventricular zone

primary visual cortex

dentate gyrus of hippocampal formation granule cell

right kidney

yolk sac

More reference expression data


More reference expression data

Gene ontology
Molecular function	nucleotide binding protein binding ATP binding ubiquitin protein ligase binding ubiquitin protein ligase activity ubiquitin-protein transferase activity NEDD8 transferase activity transferase activity
Cellular component	cytoplasm cytosol nucleoplasm
Biological process	protein neddylation positive regulation of neuron apoptotic process protein ubiquitination post-translational protein modification
Sources:Amigo / QuickGO

Databases

NCBI: entry; OMA: entry

Species

Human

Mouse


9040


22192


ENSG00000130725


ENSMUSG00000005575


P61081


P61082

RefSeq (mRNA)


NM_003969


NM_001168469 NM_001243968 NM_145578

RefSeq (protein)


NP_003960 NP_003960.1


NP_001161941 NP_001230897 NP_663553

Location (UCSC)

Chr 19: 58.56 – 58.56 Mb

Chr 7: 12.77 – 12.77 Mb

PubMed search

View/Edit Human

View/Edit Mouse

NEDD8-conjugating enzyme Ubc12 is a protein that in humans is encoded by the UBE2M gene.

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.

Interactions

UBE2M has been shown to interact with NEDD8, PRKAR1A and UBA3.

References

1 2 3 GRCh38: Ensembl release 89: ENSG00000130725 – Ensembl, May 2017
1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000005575 – Ensembl, May 2017
↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ Osaka F, Kawasaki H, Aida N, Saeki M, Chiba T, Kawashima S, Tanaka K, Kato S (Aug 1998). "A new NEDD8-ligating system for cullin-4A". Genes Dev. 12 (15): 2263–8. doi:10.1101/gad.12.15.2263. PMC 317039. PMID 9694792.
1 2 "Entrez Gene: UBE2M ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)".
↑ Gong L, Yeh ET (Apr 1999). "Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway". J. Biol. Chem. 274 (17). UNITED STATES: 12036–42. doi:10.1074/jbc.274.17.12036. ISSN 0021-9258. PMID 10207026.
↑ Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein–protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1) 89. England. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.
↑ Huang DT, Paydar A, Zhuang M, Waddell MB, Holton JM, Schulman BA (February 2005). "Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1". Mol. Cell. 17 (3): 341–50. doi:10.1016/j.molcel.2004.12.020. PMID 15694336.

Further reading

Gong L, Yeh ET (1999). "Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway". J. Biol. Chem. 274 (17): 12036–42. doi:10.1074/jbc.274.17.12036. PMID 10207026.
Chen Y, McPhie DL, Hirschberg J, Neve RL (2000). "The amyloid precursor protein-binding protein APP-BP1 drives the cell cycle through the S-M checkpoint and causes apoptosis in neurons". J. Biol. Chem. 275 (12): 8929–35. doi:10.1074/jbc.275.12.8929. PMID 10722740.
Wada H, Yeh ET, Kamitani T (2000). "A dominant-negative UBC12 mutant sequesters NEDD8 and inhibits NEDD8 conjugation in vivo". J. Biol. Chem. 275 (22): 17008–15. doi:10.1074/jbc.275.22.17008. PMID 10828074.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Tomsig JL, Snyder SL, Creutz CE (2003). "Identification of targets for calcium signaling through the copine family of proteins. Characterization of a coiled-coil copine-binding motif". J. Biol. Chem. 278 (12): 10048–54. doi:10.1074/jbc.M212632200. PMID 12522145.
Huang DT, Miller DW, Mathew R, et al. (2004). "A unique E1–E2 interaction required for optimal conjugation of the ubiquitin-like protein NEDD8". Nat. Struct. Mol. Biol. 11 (10): 927–35. doi:10.1038/nsmb826. PMC 2862556. PMID 15361859.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Huang DT, Paydar A, Zhuang M, et al. (2005). "Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1". Mol. Cell. 17 (3): 341–50. doi:10.1016/j.molcel.2004.12.020. PMID 15694336.
Li T, Santockyte R, Shen RF, et al. (2006). "A general approach for investigating enzymatic pathways and substrates for ubiquitin-like modifiers". Arch. Biochem. Biophys. 453 (1): 70–4. doi:10.1016/j.abb.2006.03.002. PMID 16620772.
Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein–protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.

v t e PDB gallery
1y8x: Structural basis for recruitment of Ubc12 by an E2-binding domain in NEDD8's E1 2nvu: Structure of APPBP1-UBA3~NEDD8-NEDD8-MgATP-Ubc12(C111A), a trapped ubiquitin-like protein activation complex

Posttranslational modification

Chaperones/
protein folding

Heat shock proteins/ Chaperonins	Hsp10/GroES Hsp27 Hsp47 HSP60/GroEL Hsp40/DnaJ A1 A2 A3 B1 B2 B11 B4 B6 B9 C1 C3 C5 C6 C7 C10 C11 C13 C14 C19 Hsp70 1A 1B 1L 2 4 4L 5 6 7 8 9 12A 14 Hsp90 α₁ α₂ β ER TRAP1
Other	Alpha crystallin Clusterin Survival of motor neuron SMN1 SMN2

Protein targeting

Ubiquitin
(ubiquitylation)

E1 Ubiquitin-activating enzyme
- UBA1
- UBA2
- UBA3
- UBA5
- UBA6
- UBA7
- ATG7
- NAE1
- SAE1

E2 Ubiquitin-conjugating enzyme
- A
- B
- C
- D1
- D2
- D3
- E1
- E2
- E3
- G1
- G2
- H
- I
- J1
- J2
- K
- L1
- L2
- L3
- L4
- L6
- M
- N
- O
- Q1
- Q2
- R1 (CDC34)
- R2
- S
- V1
- V2
- Z

E3 Ubiquitin ligase
- VHL
- Cullin
- CBL
- MDM2
- FANCL
- UBR1

Ubiquitin-like proteins
(UBL)

SUMO protein (SUMOylation)	E1 SUMO-activating enzyme SAE1 SAE2 E2 SUMO-conjugating enzyme UBC9 E3 SUMO ligase PIAS1 PIAS2 PIAS3 PIAS4
Other	ISG15 URM1 UFM1 NEDD8 (neddylation) FAT10 ATG8 ATG12 FUB1 MUB UBL5 Prokaryotic ubiquitin-like protein

Category
Commons

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