PTPN12

PTPN12
Identifiers
Aliases	PTPN12, PTP-PEST, PTPG1, protein tyrosine phosphatase, non-receptor type 12, protein tyrosine phosphatase non-receptor type 12
External IDs	OMIM: 600079; MGI: 104673; GeneCards: PTPN12
Available structures
PDB	Ortholog search: PDBe RCSB
List of PDB id codes
	5J8R
Enzyme activity
Gene location (Human)
Chr.	Chromosome 7 (human)
End	77,640,069 bp
Gene location (Mouse)
Chr.	Chromosome 5 (mouse)
End	21,055,911 bp
RNA expression pattern
	Top expressed in
	gonad; ; epithelium of colon; ; monocyte; ; right lung; ; tibial nerve; ; Achilles tendon; ; lower lobe of lung; ; upper lobe of lung; ; upper lobe of left lung; ; subcutaneous adipose tissue;
	Top expressed in
	tail of embryo; ; Gonadal ridge; ; left lung lobe; ; genital tubercle; ; stroma of bone marrow; ; trigeminal ganglion; ; abdominal wall; ; otic placode; ; hair follicle; ; dermis;
	More reference expression data
	; ;
	More reference expression data
Gene ontology
Molecular function	SH3 domain binding; phosphatase activity; protein binding; hydrolase activity; non-membrane spanning protein tyrosine phosphatase activity; phosphoprotein phosphatase activity; protein tyrosine phosphatase activity;
Cellular component	podosome; cell junction; cell projection; focal adhesion; cytoplasm; cytosol; nucleoplasm;
Biological process	tissue regeneration; dephosphorylation; protein dephosphorylation; regulation of epidermal growth factor receptor signaling pathway; peptidyl-tyrosine dephosphorylation; cellular response to epidermal growth factor stimulus; ERBB2 signaling pathway; negative regulation of ERBB signaling pathway; negative regulation of platelet-derived growth factor receptor-beta signaling pathway; cellular response to cytokine stimulus;
	Sources:Amigo / QuickGO
Orthologs
Databases	NCBI: entry; OMA: entry
	5782
	19248
	ENSG00000127947
	ENSMUSG00000028771
	Q05209
	P35831
	NM_001131008; NM_001131009; NM_002835
	NM_011203; NM_001356590; NM_001356591; NM_001356592
	NP_001124480; NP_001124481; NP_002826
	NP_035333; NP_001343519; NP_001343520; NP_001343521
	Wikidata
View/Edit Human	View/Edit Mouse

Tyrosine-protein phosphatase non-receptor type 12 is an enzyme that in humans is encoded by the PTPN12 gene.

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains a C-terminal PEST motif, which serves as a protein–protein interaction domain, and may be related to protein intracellular half-life. This PTP was found to bind and dephosphorylate the product of oncogene c-ABL, thus may play a role in oncogenesis. This PTP was shown to interact with, and dephosphorylate, various of cytoskeleton and cell adhesion molecules, such as p130 (Cas), CAKbeta/PTK2B, PSTPIP1, and paxillin, which suggested its regulatory roles in controlling cell shape and mobility.

Interactions

PTPN12 has been shown to interact with BCAR1, Grb2, PSTPIP1, TGFB1I1, Paxillin and SHC1.

References

1 2 3 GRCh38: Ensembl release 89: ENSG00000127947 – Ensembl, May 2017
1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000028771 – Ensembl, May 2017
↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ Takekawa M, Itoh F, Hinoda Y, Adachi M, Ariyama T, Inazawa J, et al. (March 1994). "Chromosomal localization of the protein tyrosine phosphatase G1 gene and characterization of the aberrant transcripts in human colon cancer cells". FEBS Letters. 339 (3): 222–228. doi:10.1016/0014-5793(94)80420-6. PMID 7509295. S2CID 4885570.
1 2 "Entrez Gene: PTPN12 protein tyrosine phosphatase, non-receptor type 12".
↑ Lin Y, Ceacareanu AC, Hassid A (August 2003). "Nitric oxide-induced inhibition of aortic smooth muscle cell motility: role of PTP-PEST and adaptor proteins p130cas and Crk". American Journal of Physiology. Heart and Circulatory Physiology. 285 (2) H710–21. doi:10.1152/ajpheart.01127.2002. PMID 12714323. S2CID 42587789.
↑ Garton AJ, Burnham MR, Bouton AH, Tonks NK (August 1997). "Association of PTP-PEST with the SH3 domain of p130cas; a novel mechanism of protein tyrosine phosphatase substrate recognition". Oncogene. 15 (8): 877–885. doi:10.1038/sj.onc.1201279. PMID 9285683.
↑ Côté JF, Charest A, Wagner J, Tremblay ML (September 1998). "Combination of gene targeting and substrate trapping to identify substrates of protein tyrosine phosphatases using PTP-PEST as a model". Biochemistry. 37 (38): 13128–13137. doi:10.1021/bi981259l. PMID 9748319.
↑ Garton AJ, Flint AJ, Tonks NK (November 1996). "Identification of p130(cas) as a substrate for the cytosolic protein tyrosine phosphatase PTP-PEST". Molecular and Cellular Biology. 16 (11): 6408–6418. doi:10.1128/MCB.16.11.6408. PMC 231642. PMID 8887669.
↑ Charest A, Wagner J, Kwan M, Tremblay ML (April 1997). "Coupling of the murine protein tyrosine phosphatase PEST to the epidermal growth factor (EGF) receptor through a Src homology 3 (SH3) domain-mediated association with Grb2". Oncogene. 14 (14): 1643–1651. doi:10.1038/sj.onc.1201008. PMID 9135065.
↑ Dowbenko D, Spencer S, Quan C, Lasky LA (January 1998). "Identification of a novel polyproline recognition site in the cytoskeletal associated protein, proline serine threonine phosphatase interacting protein". The Journal of Biological Chemistry. 273 (2): 989–996. doi:10.1074/jbc.273.2.989. PMID 9422760.
↑ Nishiya N, Iwabuchi Y, Shibanuma M, Côté JF, Tremblay ML, Nose K (April 1999). "Hic-5, a paxillin homologue, binds to the protein-tyrosine phosphatase PEST (PTP-PEST) through its LIM 3 domain". The Journal of Biological Chemistry. 274 (14): 9847–9853. doi:10.1074/jbc.274.14.9847. PMID 10092676.
↑ Shen Y, Lyons P, Cooley M, Davidson D, Veillette A, Salgia R, et al. (January 2000). "The noncatalytic domain of protein-tyrosine phosphatase-PEST targets paxillin for dephosphorylation in vivo". The Journal of Biological Chemistry. 275 (2): 1405–1413. doi:10.1074/jbc.275.2.1405. PMID 10625692.
↑ Côté JF, Turner CE, Tremblay ML (July 1999). "Intact LIM 3 and LIM 4 domains of paxillin are required for the association to a novel polyproline region (Pro 2) of protein-tyrosine phosphatase-PEST". The Journal of Biological Chemistry. 274 (29): 20550–20560. doi:10.1074/jbc.274.29.20550. PMID 10400685.
↑ Shen Y, Schneider G, Cloutier JF, Veillette A, Schaller MD (March 1998). "Direct association of protein-tyrosine phosphatase PTP-PEST with paxillin". The Journal of Biological Chemistry. 273 (11): 6474–6481. doi:10.1074/jbc.273.11.6474. PMID 9497381.
↑ Habib T, Herrera R, Decker SJ (October 1994). "Activators of protein kinase C stimulate association of Shc and the PEST tyrosine phosphatase". The Journal of Biological Chemistry. 269 (41): 25243–25246. doi:10.1016/S0021-9258(18)47237-7. PMID 7929214.
↑ Charest A, Wagner J, Jacob S, McGlade CJ, Tremblay ML (April 1996). "Phosphotyrosine-independent binding of SHC to the NPLH sequence of murine protein-tyrosine phosphatase-PEST. Evidence for extended phosphotyrosine binding/phosphotyrosine interaction domain recognition specificity". The Journal of Biological Chemistry. 271 (14): 8424–8429. doi:10.1074/jbc.271.14.8424. PMID 8626541.

Takekawa M, Itoh F, Hinoda Y, et al. (1993). "Cloning and characterization of a human cDNA encoding a novel putative cytoplasmic protein-tyrosine-phosphatase". Biochemical and Biophysical Research Communications. 189 (2): 1223–1230. doi:10.1016/0006-291X(92)92335-U. PMID 1472029.
Yi T, Cleveland JL, Ihle JN (1991). "Identification of novel protein tyrosine phosphatases of hematopoietic cells by polymerase chain reaction amplification". Blood. 78 (9): 2222–2228. doi:10.1182/blood.V78.9.2222.2222. PMID 1932742.
Garton AJ, Tonks NK (1994). "PTP-PEST: a protein tyrosine phosphatase regulated by serine phosphorylation". The EMBO Journal. 13 (16): 3763–3771. doi:10.1002/j.1460-2075.1994.tb06687.x. PMC 395289. PMID 7520867.
Habib T, Herrera R, Decker SJ (1994). "Activators of protein kinase C stimulate association of Shc and the PEST tyrosine phosphatase". The Journal of Biological Chemistry. 269 (41): 25243–25246. doi:10.1016/S0021-9258(18)47237-7. PMID 7929214.
Yang Q, Co D, Sommercorn J, Tonks NK (1993). "Cloning and expression of PTP-PEST. A novel, human, nontransmembrane protein tyrosine phosphatase". The Journal of Biological Chemistry. 268 (23): 17650. doi:10.1016/S0021-9258(19)85383-8. PMID 8349645.
Yang Q, Co D, Sommercorn J, Tonks NK (1993). "Cloning and expression of PTP-PEST. A novel, human, nontransmembrane protein tyrosine phosphatase". The Journal of Biological Chemistry. 268 (9): 6622–6628. doi:10.1016/S0021-9258(18)53296-8. PMID 8454633.
Charest A, Wagner J, Jacob S, et al. (1996). "Phosphotyrosine-independent binding of SHC to the NPLH sequence of murine protein-tyrosine phosphatase-PEST. Evidence for extended phosphotyrosine binding/phosphotyrosine interaction domain recognition specificity". The Journal of Biological Chemistry. 271 (14): 8424–8429. doi:10.1074/jbc.271.14.8424. PMID 8626541.
Garton AJ, Flint AJ, Tonks NK (1996). "Identification of p130(cas) as a substrate for the cytosolic protein tyrosine phosphatase PTP-PEST". Molecular and Cellular Biology. 16 (11): 6408–6418. doi:10.1128/MCB.16.11.6408. PMC 231642. PMID 8887669.
Charest A, Wagner J, Kwan M, Tremblay ML (1997). "Coupling of the murine protein tyrosine phosphatase PEST to the epidermal growth factor (EGF) receptor through a Src homology 3 (SH3) domain-mediated association with Grb2". Oncogene. 14 (14): 1643–1651. doi:10.1038/sj.onc.1201008. PMID 9135065.
Garton AJ, Burnham MR, Bouton AH, Tonks NK (1997). "Association of PTP-PEST with the SH3 domain of p130cas; a novel mechanism of protein tyrosine phosphatase substrate recognition". Oncogene. 15 (8): 877–885. doi:10.1038/sj.onc.1201279. PMID 9285683.
Dowbenko D, Spencer S, Quan C, Lasky LA (1998). "Identification of a novel polyproline recognition site in the cytoskeletal associated protein, proline serine threonine phosphatase interacting protein". The Journal of Biological Chemistry. 273 (2): 989–996. doi:10.1074/jbc.273.2.989. PMID 9422760.
Shen Y, Schneider G, Cloutier JF, et al. (1998). "Direct association of protein-tyrosine phosphatase PTP-PEST with paxillin". The Journal of Biological Chemistry. 273 (11): 6474–6481. doi:10.1074/jbc.273.11.6474. PMID 9497381.
Côté JF, Charest A, Wagner J, Tremblay ML (1998). "Combination of gene targeting and substrate trapping to identify substrates of protein tyrosine phosphatases using PTP-PEST as a model". Biochemistry. 37 (38): 13128–13137. doi:10.1021/bi981259l. PMID 9748319.
Wu Y, Dowbenko D, Lasky LA (1998). "PSTPIP 2, a second tyrosine phosphorylated, cytoskeletal-associated protein that binds a PEST-type protein-tyrosine phosphatase". The Journal of Biological Chemistry. 273 (46): 30487–30496. doi:10.1074/jbc.273.46.30487. PMID 9804817.
Sanger Centre T, Washington University Genome Sequencing Cente T (November 1998). "Toward a complete human genome sequence". Genome Research. 8 (11): 1097–1108. doi:10.1101/gr.8.11.1097. PMID 9847074.
Li J, Nishizawa K, An W, et al. (1999). "A cdc15-like adaptor protein (CD2BP1) interacts with the CD2 cytoplasmic domain and regulates CD2-triggered adhesion". The EMBO Journal. 17 (24): 7320–7336. doi:10.1093/emboj/17.24.7320. PMC 1171078. PMID 9857189.
Garton AJ, Tonks NK (1999). "Regulation of fibroblast motility by the protein tyrosine phosphatase PTP-PEST". The Journal of Biological Chemistry. 274 (6): 3811–3818. doi:10.1074/jbc.274.6.3811. PMID 9920935.
Angers-Loustau A, Côté JF, Charest A, et al. (1999). "Protein Tyrosine Phosphatase-PEST Regulates Focal Adhesion Disassembly, Migration, and Cytokinesis in Fibroblasts". The Journal of Cell Biology. 144 (5): 1019–1031. doi:10.1083/jcb.144.5.1019. PMC 2148201. PMID 10085298.
Nishiya N, Iwabuchi Y, Shibanuma M, et al. (1999). "Hic-5, a paxillin homologue, binds to the protein-tyrosine phosphatase PEST (PTP-PEST) through its LIM 3 domain". The Journal of Biological Chemistry. 274 (14): 9847–9853. doi:10.1074/jbc.274.14.9847. PMID 10092676.

[refGRCh38Ensembl-1] 1 2 3 GRCh38: Ensembl release 89: ENSG00000127947 – Ensembl, May 2017

[refGRCm38Ensembl-2] 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000028771 – Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid7509295-5] Takekawa M, Itoh F, Hinoda Y, Adachi M, Ariyama T, Inazawa J, et al. (March 1994). "Chromosomal localization of the protein tyrosine phosphatase G1 gene and characterization of the aberrant transcripts in human colon cancer cells". FEBS Letters. 339 (3): 222–228. doi:10.1016/0014-5793(94)80420-6. PMID 7509295. S2CID 4885570.

[entrez-6] 1 2 "Entrez Gene: PTPN12 protein tyrosine phosphatase, non-receptor type 12".

[pmid12714323-7] Lin Y, Ceacareanu AC, Hassid A (August 2003). "Nitric oxide-induced inhibition of aortic smooth muscle cell motility: role of PTP-PEST and adaptor proteins p130cas and Crk". American Journal of Physiology. Heart and Circulatory Physiology. 285 (2) H710–21. doi:10.1152/ajpheart.01127.2002. PMID 12714323. S2CID 42587789.

[pmid9285683-8] Garton AJ, Burnham MR, Bouton AH, Tonks NK (August 1997). "Association of PTP-PEST with the SH3 domain of p130cas; a novel mechanism of protein tyrosine phosphatase substrate recognition". Oncogene. 15 (8): 877–885. doi:10.1038/sj.onc.1201279. PMID 9285683.

[pmid9748319-9] Côté JF, Charest A, Wagner J, Tremblay ML (September 1998). "Combination of gene targeting and substrate trapping to identify substrates of protein tyrosine phosphatases using PTP-PEST as a model". Biochemistry. 37 (38): 13128–13137. doi:10.1021/bi981259l. PMID 9748319.

[pmid8887669-10] Garton AJ, Flint AJ, Tonks NK (November 1996). "Identification of p130(cas) as a substrate for the cytosolic protein tyrosine phosphatase PTP-PEST". Molecular and Cellular Biology. 16 (11): 6408–6418. doi:10.1128/MCB.16.11.6408. PMC 231642. PMID 8887669.

[pmid9135065-11] Charest A, Wagner J, Kwan M, Tremblay ML (April 1997). "Coupling of the murine protein tyrosine phosphatase PEST to the epidermal growth factor (EGF) receptor through a Src homology 3 (SH3) domain-mediated association with Grb2". Oncogene. 14 (14): 1643–1651. doi:10.1038/sj.onc.1201008. PMID 9135065.

[pmid9422760-12] Dowbenko D, Spencer S, Quan C, Lasky LA (January 1998). "Identification of a novel polyproline recognition site in the cytoskeletal associated protein, proline serine threonine phosphatase interacting protein". The Journal of Biological Chemistry. 273 (2): 989–996. doi:10.1074/jbc.273.2.989. PMID 9422760.

[pmid10092676-13] Nishiya N, Iwabuchi Y, Shibanuma M, Côté JF, Tremblay ML, Nose K (April 1999). "Hic-5, a paxillin homologue, binds to the protein-tyrosine phosphatase PEST (PTP-PEST) through its LIM 3 domain". The Journal of Biological Chemistry. 274 (14): 9847–9853. doi:10.1074/jbc.274.14.9847. PMID 10092676.

[pmid10625692-14] Shen Y, Lyons P, Cooley M, Davidson D, Veillette A, Salgia R, et al. (January 2000). "The noncatalytic domain of protein-tyrosine phosphatase-PEST targets paxillin for dephosphorylation in vivo". The Journal of Biological Chemistry. 275 (2): 1405–1413. doi:10.1074/jbc.275.2.1405. PMID 10625692.

[pmid10400685-15] Côté JF, Turner CE, Tremblay ML (July 1999). "Intact LIM 3 and LIM 4 domains of paxillin are required for the association to a novel polyproline region (Pro 2) of protein-tyrosine phosphatase-PEST". The Journal of Biological Chemistry. 274 (29): 20550–20560. doi:10.1074/jbc.274.29.20550. PMID 10400685.

[pmid9497381-16] Shen Y, Schneider G, Cloutier JF, Veillette A, Schaller MD (March 1998). "Direct association of protein-tyrosine phosphatase PTP-PEST with paxillin". The Journal of Biological Chemistry. 273 (11): 6474–6481. doi:10.1074/jbc.273.11.6474. PMID 9497381.

[pmid7929214-17] Habib T, Herrera R, Decker SJ (October 1994). "Activators of protein kinase C stimulate association of Shc and the PEST tyrosine phosphatase". The Journal of Biological Chemistry. 269 (41): 25243–25246. doi:10.1016/S0021-9258(18)47237-7. PMID 7929214.

[pmid8626541-18] Charest A, Wagner J, Jacob S, McGlade CJ, Tremblay ML (April 1996). "Phosphotyrosine-independent binding of SHC to the NPLH sequence of murine protein-tyrosine phosphatase-PEST. Evidence for extended phosphotyrosine binding/phosphotyrosine interaction domain recognition specificity". The Journal of Biological Chemistry. 271 (14): 8424–8429. doi:10.1074/jbc.271.14.8424. PMID 8626541.

PTPN12

Interactions

References

Further reading